秋水仙碱对肝纤维化大鼠肝脏基质金属蛋白酶-1及其抑制因子-1表达的影响  

Effects of colchicine on the expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in the livers of rats with hepatic fibrosis

作　　者：杨长青[1] 胡国龄[2] 周文红[2] 谭德明[2] 张铮[2] 

机构地区：[1]上海医科大学中山医院消化内科工作 [2]湖南医科大学湘雅医院传染病研究所

出　　处：《中华传染病杂志》2000年第3期176-179,共4页Chinese Journal of Infectious Diseases

摘　　要：目的　观察秋水仙碱对纤维化肝脏基质金属蛋白酶 1(MMP 1)、基质金属蛋白酶组织抑制因子 1(TIMP 1)表达的影响 ,从胶原降解的角度探讨秋水仙碱对肝纤维化有无逆转作用及可能存在的机制。方法　制备免疫性大鼠肝纤维化模型 ,并给予秋水仙碱治疗 ;通过RT PCR检测MMP 1、TIMP 1的表达 ,并作Ⅰ、Ⅲ型胶原的免疫组化以及Masson胶原染色。结果　发现秋水仙碱对肝纤维化大鼠MMP 1的表达无明显影响 (P >0 .0 5 ) ,但可以抑制TIMP 1的表达 (P <0 .0 5 ) ,促进Ⅰ、Ⅲ型胶原的降解 (P <0 .0 5 ) ;然而在病理形态学的观察中 ,未发现秋水仙碱治疗组与肝纤维化模型组之间存在的显著性差异 (P >0 .0 5 )。结论　秋水仙碱可以抑制纤维化肝脏TIMP 1的表达 ,从而增强间质胶原酶的活性 ,促进Ⅰ、Ⅲ型胶原的降解 ,产生抗肝纤维化的作用 。Objective Through observing the effects of colchicine on the expression of matrix metalloproteinase 1 (MMP 1) and Tissue Inhibitior of Metalloproteinase 1 (TIMP 1) in the livers of rats with hepatic fibrosis, to study the impact of colchicine on liver fibrosis and its possible mechanism in the way of degradation of collagens. Methods Rat′s model of liver fibrosis was prepared by immune injuring, and treated with colchicine. The effects were observed by quantitative reverse transcription polymerase chain reaction (RT PCR) assaying the expression of MMP 1 and TIMP 1. Meanwhile, the expressions of type Ⅰ and type Ⅲ collagen were detected by Masson staining. Results It was found that the colchicine had no effect on the expression of MMP 1( P >0.05), but could inhibit the expression of TIMP 1( P <0.05) and accelerate the degradation of both collagen Ⅰ and collagen Ⅲ ( P <0.01). However there was no obvious significant difference between colchicine treated group and untreated group according to the results of pathological assay ( P >0.05).Conclusion Colchicine can inhibit the expression of TIMP 1 in the liver of rats with hepatic fibrosis, enhance the activation of MMP 1, and increase the destruction of type Ⅰ and Ⅲ collagen, but limitedly.

关 键 词：肝纤维化 秋水仙碱 MMP-1 TIMP-1 

分 类 号：R575.2[医药卫生—消化系统]