响应面法优化光合细菌Rhodospirillum Rubrum S1培养条件  

Optimization of Culture Conditions for Photosynthetic Bacteria Rhodospirillum Rubrum S1 by Response Surface Methodology

作　　者：潘艳[1,2] 李师豪[1] 赵兴红[1] 王恩思[1] 侯阿澧[1] 

机构地区：[1]吉林大学生命科学学院,长春130012 [2]吉林工商学院食品工程分院,长春130062

出　　处：《中国调味品》2013年第8期34-37,42,共5页China Condiment

摘　　要：文章首先用单因素法分别对光合细菌Rhodospirillum Rubrum S1的培养条件——温度、光照强度、接种量进行了考察,确定了最佳培养条件:温度30℃,光照强度3000lx,接种量15%。在此基础上,采用中心组合试验设计结合响应面分析法(Response Surface Methodology,RSM)对影响光合细菌Rhodospirillum Rubrum S1的发酵条件进行进一步优化。结果表明,主要影响因素的最佳条件:培养温度32.54℃,光照强度2995.85lx,接种量15.80%,光合细菌Rhodospirillum Rubrum S1的类胡萝卜素产量为16.72mg/L,与理论值16.86mg/L相差0.83%。因此,利用响应面分析法对光合细菌Rhodospirillum Rubrum S1的培养条件进行优化合理可行。In the paper, the single factor method is used to study the eulture conditions of photosyn- thetic bacteria （PSB） Rhodospirillum Rubrum S1, including temperature, light intensity and inocula- tion quantity. The optimal culture conditions are as follows： temperature of 30 ℃, light intensity of 3000 Ix and inoculation quantity of 15%. On the basis of the above conditions, Response Surface Methodology （RSM） is adopted for further optimization of other factors that affect fermentation con- ditions of PSB Rhodospirillum Rubrum S1. The results show that the optimal temperature is 32.54 ℃, light intensity is 2995.85 lx and inoculation quantity is 15.80%. Besides, the output of carotenoid is 16.72 rag/L, which differs from the theoretical value 16.86 mg/L by 0.83%. As a con- sequence, RSM to optimize the culture conditions of PSB S1 is reasonable and feasible.

关 键 词：光合细菌RhodospirillumRubrum S1 培养条件 响应面法 

分 类 号：TS201.1[轻工技术与工程—食品科学]