MDM2基因在鼻咽癌中的表达及其与p53蛋白表达、EB病毒潜伏感染的关系  

MDM2 gene expression in nasopharyngeal carcinoma and its relationship with p53 protein expression and EB virus latent infection

作　　者：罗均利[1] 肖健云[1] 田勇泉[1] 赵素萍[1] 刘季威[1] 陶正德[1] 

机构地区：[1]湖南医科大学湘雅医院耳鼻咽喉科研究室,长沙410008

出　　处：《临床耳鼻咽喉科杂志》2000年第11期507-509,共3页Journal of Clinical Otorhinolaryngology

摘　　要：目的 :探讨 MDM2基因在鼻咽癌 (NPC)中的表达情况及其与 p5 3蛋白表达、EB病毒 (EBV )潜伏感染的关系。方法 :运用非同位素原位杂交、免疫组化和多聚酶链反应技术对 46例 NPC、12例慢性鼻咽炎症粘膜(CINE)分别进行 MDM2 m RNA、MDM2蛋白、p5 3蛋白及 EBV- DNA的检测。结果 :46例 NPC中 ,14例为MDM2 m RNA及 MDM2蛋白高表达 ,38例为 p5 3蛋白阳性 ,43例为 EBV- DNA阳性 ;12例 CINE均无 MDM2m RNA、MDM2蛋白及 p5 3蛋白表达 ;MDM2的高表达与 p5 3蛋白的表达相关 (P <0 .0 5 ) ,而与 EBV- DNA无关。结论 :MDM2可能通过与 p5 3蛋白相互作用而在 NPC的发生过程中起着重要作用。Objective:To define the expression levels of MDM2 gene in nasopharyngeal carcinoma (NPC) and its relationship with p53 protein expression and EB virus latent infection.Method:[WT5”,6BZ]MDM2 gene expression at mRNA and protein levels,p53 protein and EB virus DNA were detected by nonradioactive in situ hybridization(ISH),immunohistochemistry(IHC) and polymerase chain reaction (PCR) separately in 46 cases of NPC tissues and 12 cases of chronic inflammation of nasopharyngeal epithelium(CINE).Result:Fourteen cases of NPC showed MDM2 mRNA and protein overexpression,38 cases were p53 protein positive,and 43 cases were EBV-DNA positive.Neither MDM2 nor p53 protein was expressed in any case of CINE.MDM2 expression was significantly related to p53 protein expression(P< 0.05 ),but not to EB virus latent infection in NPC.Conclusion:MDM2 gene may play an important role in the pathogenesis of NPC through interacting with p53 protein.

关 键 词：鼻咽癌 MDM2基因 表达 P53蛋白 EB病毒潜伏感染 

分 类 号：R739.630.2[医药卫生—肿瘤]