人骨髓瘤细胞Sko-007中的白细胞介素6信号转导途径研究  

Study of IL-6 signal transduction pathway in a human multiple myeloma cell line-Sko 007

作　　者：宋伦[1] 黎燕[1] 沈倍奋[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850

出　　处：《中华血液学杂志》2000年第11期569-571,共3页Chinese Journal of Hematology

基　　金：国家杰出青年科学基金(39925019)资助项目

摘　　要：目的　研究人骨髓瘤细胞系Sko 0 0 7中的白细胞介素 6 (IL 6 )信号转导途径。方法　采用凝胶阻滞电泳和免疫沉淀方法观察IL 6刺激前后Sko 0 0 7细胞中IL 6相关转录因子STAT3、NF IL 6、NF kB和参与IL 6信号转导功能的蛋白激酶ERK、Jak1的诱导活化状态并确定该细胞中的IL 6信号转导通路。结果　转录因子STAT3、NF IL 6和蛋白激酶ERK、Jak1都能在Sko 0 0 7细胞中被IL 6诱导激活。结论　Jak/STAT和Ras/NF IL 6途径的活化介导了IL 6在Sko 0 0 7细胞中的信号转导功能。s Objective To investigate the IL 6 signal transduction pathway in a human multiple myeloma (MM) cell line—Sko 007. Methods The electrophoretic mobility shift assay (EMSA) and immunoprecipitation were used to detect the activation of IL 6 related transcription factors(TFs)——STAT3,NF IL 6,NF kB and protein kinase ERK,Jak1 in Sko 007 cells stimulated by IL 6. Results Both the transcription factors STAT3,NF IL 6 and protein kinases Jak1,ERK were activated by IL 6 in Sko 007 cells. Conclusion The biological function of IL 6 on Sko 007 cells is mediated by the activation of Jak/STAT and Ras/NF IL 6 signal transduction pathways.

关 键 词：多发性骨髓瘤 白细胞介素6 信号转导 

分 类 号：R733.3[医药卫生—肿瘤]