人多发性骨髓瘤细胞syndecan-1的表达、分泌及调节  

The expression, secretion and regulation of membrane soluble syndecan 1 in human multiple myeloma cells

作　　者：李新燕[1] 陆肇阳[1] 张学光[1] BKlein 

机构地区：[1]苏州大学免疫研究室,215007 [2]法国蒙彼利埃总医院

出　　处：《中华血液学杂志》2000年第11期572-576,共5页Chinese Journal of Hematology

基　　金：国家自然科学青年基金资助项目! (3970 0 130 )

摘　　要：目的　研究蛋白聚糖家族成员syndecan 1分子在人多发性骨髓瘤 (MM)细胞表面的表达和可溶型syndecan 1分子的分泌及其调节 ,以期探讨syndecan 1与人MM发生、发展的相关性。方法　用免疫荧光标记流式细胞仪分析人MM肿瘤细胞表面syndecan 1的表达 ;采用ELISA法检测人MM细胞培养上清可溶型syndecan 1的分泌水平。结果　①人MM细胞表面表达高水平的syndecan 1分子 ,随着肿瘤细胞的凋亡 ,细胞表面syndecan 1分子表达丢失 ;②大多数骨髓瘤细胞分泌可溶型syndecan 1分子 ,其分泌水平与细胞状态有关 ,而与膜表面syndecan 1分子的密度无关 ,而且可溶型syndecan 1的产生不依赖于血清的存在 ;③蛋白合成抑制剂放线菌酮抑制可溶型syndecan 1分子的产生 ,丝氨酸蛋白酶抑制剂antipain对syndecan 1分子的分泌无影响。结论　syndecan 1分子作为多种生长因子、细胞因子以及肝素样结合生长因子的受体 ,在MM的病理过程中 ,对于骨髓瘤细胞的生长。Objective To analyze the expression, secretion and regulation of membrane soluble syndecan 1 in human multiple myeloma cells,and to explore the relationship between syndecan 1 and the development of human multiple myeloma. Methods Syndecan 1 expression on the surface of malignant plasma cells was analyzed by immuno staining, the levels of soluble syndecan 1 in the supernatant of cultured tumor cells by ELISA. Results ①Human multiple myeloma cells expressed high levels of syndecan 1. The density of syndecan 1 varied from 170?000 to 800?000 molecules per cell. The expression of syndecan 1 lost rapidly as the cells underwent apoptosis. ②Most myeloma cells produced soluble syndecan 1, the rate of production did not correlate with the density of membrane syndecan 1. Moreover, the production of soluble syndecan 1 did not require serum. ③The protein synthesis inhibitor cycloheximide inhibited the production of syndecan 1. The serine protease inhibitor antipain did not affect the secretion of syndecan 1. Conclusion As a receptor of many growth factors, cytokines and heparin like growth factors, syndecan 1 might play an important role in tumor cell growth and homing in multiple myeloma development.

关 键 词：多发性骨髓瘤 syndecan-1分子 膜型 可溶型 

分 类 号：R733.3[医药卫生—肿瘤]