柑橘籽类柠檬苦素提取工艺及其体外抗肿瘤研究  

Research on Subcritical Water Extraction of Limonoids from Citrus Seed by Response Surface Methodology and Anti-tumor

作　　者：令红艳[1] 封家福[1] 张知贵[1] 

机构地区：[1]乐山职业技术学院药学系,乐山614000

出　　处：《食品工业》2013年第8期57-59,共3页The Food Industry

摘　　要：以柑橘籽为原料,研究柑橘籽类柠檬苦素亚临界水萃取提取工艺及体外抗肿瘤作用。采用亚临界水提取柑橘籽类柠檬苦素,利用响应面分析法优化其提取工艺,并对柑橘籽类柠檬苦素对肿瘤细胞株抑制作用进行分析。结果表明柑橘籽类柠檬苦素萃取最佳工艺条件为萃取时间为18.8 min,萃取温度为188.3℃,物料粒径为71.3目(210μm),液料比值为23.3(mL/g)。而且类柠檬苦素对肿瘤细胞株有较强的抑制作用。The extraction technology and anti-tumor of limonoids from citrus seed were discussed. Limonoids from citrus seed was extracted by subcritical water, the optimum extraction conditions of limonoids from citrus seed were determined by re- sponse surface methodology, inbibitional effects of limonoids to cancer cell lines were analyzed. The optimum extraction con- ditions were as follows： extraction time was 18.8 min, the extraction temperature was 188.3 ℃, the particle size of the material was 71.3 meshes （210 μm）, the ratio of solution and material was 23.3 （mL/g）, and limonoids from citrus seed had strong inhibition effects on cancer cell lines.

关 键 词：类柠檬苦素 亚临界水萃取 体外抗肿瘤 响应面分析法 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]