中药秦艽提取物抑制人晶状体上皮细胞迁移的研究  

Inhibition of the human lens epithelial cells migration by gentiana macrophylla pall extract

作　　者：刘玉福[1] 周毅[1] 刘宏伟[1] 

机构地区：[1]首都医科大学附属北京同仁医院北京市眼科研究所,北京100005

出　　处：《中国现代医生》2013年第24期1-3,共3页China Modern Doctor

基　　金：十二五"重大新药创制"科技重大专项基金资助项目(2011ZX09302-007-05)

摘　　要：目的为了验证中药秦艽提取物抑制培养的人晶状体上皮细胞迁移的作用,寻找防治后发障的新药物。方法晶状体上皮细胞长成单层后,分别加入1000、500、250、125和62.5 mg/L的秦艽提取物,培养24 h,观察细胞毒性,确定药物的半数毒性浓度。细胞用1 mL加样器枪头划痕,分别加入100、10、1、0.1 mg/L秦艽提取物,培养24 h。对细胞进行照像,测出实验前后的细胞痕的宽度,计算出细胞迁移距离,测定药物的半数有效浓度及计算秦艽提取物的治疗指数。结果秦艽提取物半数细胞毒性浓度为421.7 mg/L。以秦艽提取物浓度对数与迁移距离做回归分析,结果显示相关系数为-0.966,有显著性差异(P=0.034)。秦艽提取物的半数有效治疗浓度为0.38 mg/L。秦艽提取物治疗指数为1110。结论秦艽提取物可抑制晶状体上皮细胞的迁移,具有低毒、高效的特点。Objective To verify the gentiana macrophylla pall extract inhibiting cultured human lens epithelial cell migration, looking for new drugs for prevention and treatment of after-cataract. Methods The lens epithelial cells grew into monolayer, added 1000,500,250,125 and 62.5 mg/L gentiana macrophylla pall extracts,and cultured for 24 hours. The median toxic concentration （IC50） was determined by observation of cell toxicity. The cells were scratched by l mL pipette gun tip, added 100,10,1,0.1 mg/L gentiana macrophylla pall extracts, and cultured for 24 hours. The cell trace widths were measured before and after the experiment, and cell migration distances were calculated for de- termination of median effective concentration（ED50）. The treatment index（TI） of gentiana macrophylta pall extract was calculated. Results The median toxic concentration（IC50） of gentiana macrophylla pall was 421.7 mg/L. The regression analysis was done by the logarithmlogarithmlogarithm of gentiana macrophyUa pall extract concentration and migration distance, the results showed that the correlation coefficient was -0.966, there was significant difference （P = 0.034）.The median effective concentration（EC50） was 0.38 mg/L. The treatment index（TI） was 1110. Conclusion Gentiana macrophylla pall extract can inhibit lens epithelial cells migration. Gentiana macrophylla pall extract has the charac- teristics of low toxicity, high efficiency, is expected to become the effective drugs for prevention and treatment of after- cataract.

关 键 词：秦艽提取物 晶状体上皮细胞 迁移 后发障 

分 类 号：R776.1[医药卫生—眼科]