泻火化瘀通窍法改善耳蜗缺血再灌注损伤的实验研究  

Experimental Research on Method of Reducing Fire and Resolving Stasis to Dredge Orifice and Improve Cochlea Ischemia Reperfusion Injury

作　　者：冷辉[1] 孙海波[1] 王爱平[1] 曲中源[1] 马梽轩[1] 

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中华中医药学刊》2013年第8期1670-1673,I0007,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省教育厅资助项目(2009T067)

摘　　要：目的:探讨泻火化瘀通窍法对耳蜗组织缺血再灌注损伤iNOSmRNA和MMP-2mRNA表达的影响。方法:健康豚鼠60只,随机分成5组,每组12只,即正常组、模型组、行气组、活血化瘀组、泻火化瘀通窍组,除正常组外,其余各组分别进行光化学诱导法造模,成功后正常组:予0.9%生理盐水4 mL/(只.d)灌胃;模型组:予0.9%生理盐水4 mL/(只.d)灌胃;行气组:予行气中药4 mL/(只.d)灌胃(含生药1 g);活血化瘀组:予活血化瘀药物4 mL/(只.d)灌胃(含生药2 g),共连续给药10天;泻火化瘀通窍组,予泻火化瘀通窍药物4 mL/(只.d)灌胃(含生药4 g),各组均每日分2次给药,共连续给药10 d。10 d后取耳蜗组织行实时荧光定量PCR检测iNOSmRNA及MMP-2mRNA。结果:正常组与各治疗组iNOSmRNA的表达均明显低于模型组P<0.05,且泻火化瘀通窍组iNOSmRNA的表达量与活血化瘀组接近P>0.05,模型组iNOSmRNA的表达约是行气组的1.3倍,约是活血化瘀组的4.7倍,约是泻火化瘀通窍组的4.6倍;各治疗组MMP-2mRNA的表达均明显低于模型组P<0.05,模型组MMP-2mRNA的表达是是行气组的1.5倍;是活血化瘀组的2.4倍;是泻火化瘀通窍组的7.3倍。结论:①泻火化瘀通窍法与活血化瘀法均能够抑制iNOS的表达,保护耳蜗组织的缺血损伤,明显优于行气法。②泻火化瘀通窍法、活血化瘀法与行气法均能够抑制MMP-2的表达,且泻火化瘀通窍法作用最强。Objective： To explore the effect of method of reducing fire and resolving stasis to dredge the orifice （RRDO） on iNOSmRNA and MMP- 2mRNA expressions of cochlea ischemia reperfusion injury. Method：60 healthy guinea pigs were randomly divided into 5 groups. Animal model and grouping： photochemical induction method was used in modeling, then the guinea pig models were divided into five groups, namely normal group, model group, qi - promo- ting group, blood - activating group and RRDO group, with 12 guinea pigs in each group. Experimental method： 2 mL of 0.9% normal saline twice a day for the normal group ; 2 mL of 0.9% normal saline twice a day for the model group 12 h after the operation ; and different treatments for the rest three groups. The specimens of the guinea pigs were sampled after quick decapitation on the 2nd day after administration of anesthesia, for RRDO group： 12 h after the operation, adminis- tration of the RRDO decoction, 2 mL, twice a day （amounting to 1 g/d for the raw medicine） ; for the blood - activating group： administration of the ingredients activating blood to resolve stasis in the RRDO decoction, 2 mL, twice a day （a- mounting to 2 g/d for the raw medicine） ; for the qi -promoting group： administration of the ingredients the qi -promo- ting simply in the RRDO decoction, 2 mL, twice a day （amounting to 4 g/d for the raw medicine）. The course of treat- ment consisted of 10 days. Expressions of cochlear iNOSmRNA and MMP - 2mRNA were determined by using Real - Time PCR after 10 days. Results： In the normal group and all the 3 treatment groups, the expressions of iNOSmRNA were significantly lower than that in the model group （P 〈 0. 05）. The expression of iNOSmRNA in the RRDO group was simi- lar with that in the blood - activating group （P 〉 0. 05 ）. The expression of iNOSmRNA in the model group was about 1.3 times what it was in the qi - promoting group, about 4.7 times what it was in the blood - activating group, and about 4.6 times what it was i

关 键 词：泻火化瘀通窍法 耳蜗 缺血再灌注损伤 

分 类 号：R-332[医药卫生]