温胰蛋白酶消化法和冷消化法培养乳鼠心肌细胞的比较  

Comparison of Cardiomyocyte Culture Obtained by Temperature of Trypsin Digestion and Cold Digestion from Newborn Rats

作　　者：王绮雯[1] 吴启端[2] 陈奕芝[2] 

机构地区：[1]广州医学院第二附属医院外科实验室,广东广州510260 [2]广州中医药大学第一附属医院实验中心,广东广州510405

出　　处：《中国医药指南》2013年第20期401-403,共3页Guide of China Medicine

基　　金：2010年广州市中医药中西医结合科研课题(2010A25)

摘　　要：目的探索一种经济、简单易行的心肌细胞培养方法。方法选用新生乳大鼠心肌细胞进行0.25%胰蛋白酶温消化法和冷消化法。结果与温消化相比,冷消化可获得较高的细胞存活率、24h生存率,而且在开始贴壁生长、出现搏动、形成所谓功能性合体细胞的时间和搏动频率等方面,冷消化法培养的心肌细胞都优于温消化法。结论虽然温胰蛋白酶消化法应用广泛,但冷消化能产生大量可存活细胞且较省力、经济、实验要求条件不高,故冷消化法优于温消化法。Objective Exploration of an economic and simple myocardial cell culture method. Methods 0.25% trypsin temperature digestion and cold digestion for culturing cardiomyoeytes from newborn rats. Results Compared with temperature digestion, cold digestion can obtain higher cell survival rate and 24h survival rate, at the beginning of adherent growth, pulsatile,forming the so-called functional syncytium time and pulse frequency, cold digestion in cultured myocardial cells are superior to temperature digestion method. Conclusion Although the temperature of trypsin digestion is widely used, but cold digestion to produce a large number of viable cells and less effort, economic, and experimental conditions are not required, hence the digestion method is superior to cold temperature digestion.

关 键 词：细胞培养 心肌细胞 温消化法 冷消化法 

分 类 号：R-33[医药卫生]