离子液体中酶促转酯化合成咖啡酸苯乙酯的新工艺  

New process for enzymatic synthesis of caffeic acid phenethyl ester via transesterification in ionic liquid

作　　者：张磊霞[1] 王俊[1] 顾双双[1] 肖维[1] 李晶[1] 吴福安[1,2] 郭锡杰[1,2] 

机构地区：[1]江苏科技大学生物与化学工程学院,江苏镇江212018 [2]中国农业科学院蚕业研究所,江苏镇江212018

出　　处：《江苏科技大学学报（自然科学版）》2013年第3期295-299,共5页Journal of Jiangsu University of Science and Technology:Natural Science Edition

基　　金：国家自然科学基金项目(21206061);江苏省自然科学基金项目(BK2009213);中国博士后基金项目(2012M510124);江苏省高校"青蓝工程"优秀青年骨干教师资助计划项目;江苏科技大学博士启动项目(35211002);江苏科技大学研究生科技创新计划项目

摘　　要：为提高抗肿瘤活性化合物咖啡酸苯乙酯的生物合成效率,建立离子液体中酶促转酯化合成新工艺.文中以咖啡酸甲酯和苯乙醇为原料,以脂肪酶和离子液体分别作为催化剂和反应介质,以咖啡酸苯乙酯得率为指标,考察了离子液体、脂肪酶、反应温度、底物摩尔比和酶用量等对咖啡酸苯乙酯得率的影响.结果表明:Novozym 435和[Bmim][Tf2N]分别为最佳的催化剂和反应介质;当反应温度90℃、醇酯摩尔比20∶1、酶酯质量比10∶1时,转酯化反应24 h,咖啡酸苯乙酯得率达66.40%.因此,离子液体中酶促转酯化合成咖啡酸苯乙酯相比直接酯化合成法得率高,耗时短.To improve the biosynthesis efficiency of caffeic acid phenethyl ester （CAPE） with the antitumor ac- tivity, a new process for lipase-catalyzed transesterification in ionic liquid was established. In the present study, methyl caffeate （MC） and phenylethanol （PE） were used as substrates, lipase and ionic liquid were used as cat- alyst and reaction media, the CAPE yield was used as an evaluation index, the effects of several factors including ionic liquid, lipase, reaction temperature, substrate molar ratio and enzyme dosage on CAPE yield were as- sessed, respectively. The results indicated that Novozym 435 and [ Bmim] [ Tf2N] were selected as the optimal catalyst and reaction media, respectively. The highest CAPE yield achieved 66.40 % when the reaction after 24 h under the optimal condition as follows ： temperature 90 ℃, molar ratio of PE to MC 20： 1, mass ratio of No- vozym 435 to MC 10： 1. Therefore, the lipase-catalyzed synthesis of CAPE via transesterification in ionic liquid has higher yield and shorter time than direct esterification.

关 键 词：咖啡酸苯乙酯 酶促合成 离子液体 转酯化反应 

分 类 号：Q599[生物学—生物化学]