HPLC检测发酵液中二羟基丙酮和甘油的含量  

Quantitative Determination of Dihydroxyacetone and Glycerol in the Fermentation Broth by HPLC

作　　者：谢光蓉[1] 

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京210009

出　　处：《食品与发酵科技》2013年第4期70-74,80,共6页Food and Fermentation Science & Technology

摘　　要：应用高效液相色谱法检测发酵过程中发酵产物二羟基丙酮和底物甘油浓度。在流动相乙腈、纯净水的比例为85∶15(v∶v),流速为1mL/min,色谱柱为Hedera-NH2的条件下,用紫外检测器在271nm处检测DHA,用示差折光检测器检测甘油。高效液相色谱法检测DHA的线性范围为2.0-12.0mg/mL,相关系数(r)为0.9996,检测甘油的线性范围为0.1-1.0mg/mL,相关系数(r)为0.9998。该方法应用于二羟基丙酮发酵过程中底物和产物的检测,该方法不受培养基中不同碳源(葡萄糖、蔗糖、山梨醇、乳糖、甘油),不同氮源(蛋白胨、酵母膏、酵母粉、营养肉汤)以及菌体未完全利用的培养基成分、菌体生长过程中产生的其它代谢产物的影响,该方法具有较好的通用性。High performance liquid chromatography（HPLC） was applied to determinate dihydroxyacetone（DHA） and glycerol in the fermentation broth respectively.The mobile phase is composed of acetonitrile-water（85∶15,V∶V）.The analytes were separated on a Hedera-NH2 column with at a flow rate of 1mL/min.DHA was detected by UV detector at 271nm and glycerol was detected by refractometer.The linearity range for the determination of DHA was 2.00-12.00mg/mL with a correlation coefficient（r） of 0.9996.The linearity range for the determination of glycerol was 0.1-1.0mg/mL with a correlation coefficient（r） of 0.9998.The methods were applicable for DHA and glycerol determination in the fermentation process without interference from other constitutes in the fermentation broth.

关 键 词：高效液相色谱法 二羟基丙酮 甘油 

分 类 号：TQ920[轻工技术与工程—发酵工程] TS207.3