慢性应激性抑郁对大鼠皮下种植性肿瘤Walker256的影响及其作用机制  

Effect of Chronic Stress-induced Depression on Subcutaneously Implanted Walker 256 Tumors in Rats and Its Mechanism

作　　者：王双彪[1] 姜达[2] 张增叶[3] 

机构地区：[1]河北省廊坊市人民医院医务部,河北廊坊065000 [2]河北医科大学第四医院肿瘤内科,石家庄050011 [3]河北医科大学第三医院肿瘤内科,石家庄050051

出　　处：《南昌大学学报（医学版）》2013年第5期8-12,16,共6页Journal of Nanchang University：Medical Sciences

摘　　要：目的观察不同程度的慢性应激性抑郁对大鼠皮下种植性肿瘤Walker256的影响,并探讨其可能的作用机制。方法构建大鼠慢性应激性抑郁模型及皮下种植性肿瘤模型,将48只SD雌性大鼠按随机数字表法分为4组,每组12只。单纯种瘤组(A组)只给予大鼠接种Walker256癌肉瘤细胞,种瘤前后均不给予应激;种瘤后给予应激性抑郁组(B组)接种Walker256癌肉瘤细胞后给予16d各种不同应激;种瘤前给予应激性抑郁组(C组)接种Walker256癌肉瘤细胞前给予16d各种不同的应激;种瘤前后均给予应激性抑郁组(D组)接种Walker256癌肉瘤细胞前后均给予16d各种不同的应激。观察各组大鼠接种Walker256癌肉瘤细胞后的生存期,检测各组大鼠行为学指标以及脾重、瘤重、血清皮质醇、白细胞介素-6(IL-6)等指标。结果大鼠行为学指标的比较:与A组相比,B、C、D组大鼠活动时间、活动总路程、中央区域活动时间显著减少(P<0.05),休息时间、四周区域活动时间显著增加(P<0.05);D组与B、C组相比,活动总路程亦显著减少(P<0.05),而活动时间、中央区域活动时间减少、休息时间、四周区域活动时间延长,但其差异无统计学意义(P>0.05)。大鼠生存期的比较:与A组相比,D组明显缩短(P<0.05),而其他各组大鼠差异无统计学意义(P>0.05)。大鼠脾重的比较:与A组相比,D组明显减轻(P<0.05),其余各组差异无统计学意义(P>0.05)。大鼠瘤重的比较:A、B、C、D组差异无统计学意义(P>0.05)。大鼠血清皮质醇的比较:与A组相比,B、C、D组显著升高(P<0.05);D组较B、C组亦显著升高(P<0.05)。各组大鼠血清IL-6的比较:与A组相比,D组显著升高(P<0.05),其余各组差异无统计学意义(P>0.05)。结论慢性应激能使大鼠表现出较明显的抑郁症状,自发活动减少,探究行为降低,且应激性抑郁时间越长,抑郁症状越严重。较长时间的慢性应激性抑郁可显著缩短荷瘤大鼠的生存期,�Objective To observe the effect of chronic stress-induced depression on subcutaneously implanted Walker 256 tumors in rats,and to explore its possible mechanism.Methods Rat models of chronic stress-induced depression and subcutaneous implanted tumors were established in this study.Forty-eight female SD rats were randomly divided into four groups,with 12 rats in each group.Group A was only inoculated with Walker 256 carcinosarcoma cells,without stress before and after inoculation;Group B was given various stress treatments for 16 days after inoculation with Walker 256 carcinosarcoma cells;Group C was given various stress treatments for 16 days before inoculation with Walker 256 carcinosarcoma cells;Group D was given various stress treatments for 16 days both before and after inoculation with Walker 256 carcinosarcoma cells.The survival,behavior indexes,spleen weight,tumor weight,serum cortisol levels and interleukin-6（IL-6） levels were measured after inoculation.Results Compared with group A,movement time,movement distance and central regional movement time significantly decreased but rest time and peripheral regional movement time significantly increased in group B,C and D（P〈0.05）.Compared with group B or C,movement distance significantly decreased in group D（P〈0.05）.In addition,movement time,rest time,central regional movement time and peripheral regional movement time in group D were longer than those in group B or C,but differences were not significant（P〈0.05）.Compared with group A,survival time and spleen weight obviously decreased but IL-6 levels obviously increased in group D（P〈0.05）.However,no significant differences were found among group A,B and C（P〈0.05）.Compared with group A,serum cortisol levels significantly increased in group B,C and D（P〈0.05）.Compared with group D,serum cortisol levels significantly increased in group C and D（P〈0.05）.There were no significant differences in tumor weight among the four groups（P〈0.05）.Conclusion Chronic stress can i

关 键 词：应激性抑郁 慢性 种植性肿瘤 动物 实验 大鼠 

分 类 号：R-332[医药卫生]