东亚钳蝎毒素多肽BmK AS-1对大鼠皮肤痛觉的影响  

Effect of BmK AS-1, polypeptide purified from Chinese Buthus martensi Karsch (BmK) toxin, on rat cutaneous nociception

作　　者：肖杭[1] 毛霞[1] 谭智勇[2] 石云[1] 张建伟[2] 程健[1] 赵志奇[2] 吉永华[2] 

机构地区：[1]南京医科大学毒理学研究所,江苏南京210029 [2]中国科学院上海生理研究所,上海200031

出　　处：《中国药理学与毒理学杂志》2000年第4期283-286,共4页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金项目!(39870 70 2 );江苏省自然科学基金项目!(BK95 0 76 30 1)&&

摘　　要：观察东亚钳蝎 ( Bm K)毒素纯化组分 Bm K F-1 - 3,Bm K F- 1 - 3- 2和 Bm K AS- 1 ( Bm K F- 1 - 3- 2 - 1 )中枢和外周给药对大鼠皮肤痛觉的影响 .方法采用局部皮肤感受野给药 ,以强电流刺激大鼠后肢趾部诱发半膜半腱肌发放 C反应 ,观察对外周神经系统的镇痛作用 ;经脊髓蛛网膜下腔给药 ,以大鼠足跖辐射热痛阈的变化为中枢镇痛效应的观察指标 .实验结果显示 Bm K F- 1 - 3,Bm K F- 1 - 3- 2和 Bm KAS- 1抑制 50 % C反应的剂量为 40 ,2 8.3和 1 0μg,且抑制作用不能被纳洛酮翻转 ;Bm K F- 1 - 3ith无明显提高大鼠足跖辐射热痛阈的作用 ,而 Bm KAS- 1 ith则可显著提高大鼠足跖辐射热痛阈 ,其提高 1 50 %痛阈的剂量约为 1 .2μg,纳洛酮同样对Bm K AS- 1的中枢镇痛效应无翻转作用 .结果提示东亚钳蝎毒素纯化组分 Bm K AS- 1可提高大鼠皮肤痛阈 ,其作用机理有别于阿片肽类物质 .The effect of the purification of Buthus martensi Karsch (BmK) toxin including BmK F 1 3, BmK F 1 3 2 and BmK AS 1(BmK F 1 3 2 1) on the senation of pain was observed. The C response of semimembranous and semitendinous myoelectricity evoked by stimulation to rat intraplanta with high intensive currents was used to illustrate the peripheral analgesic effect of BmK when given in local cutaneous sensory fields. And the central analgesic effect of BmK was also investigated when applied intrathecally (ith) on the basis of the change in pain threshold of rat radiation heat. The result showed that 50% C response was suppressed in a naloxone irreversible manner by 40, 28.3 and 10 μg of BmK F 1 3, BmK F 1 3 2 and BmK AS 1, respectively. No effect of BmK F 1 3 on pain threshold of rat radiation heat was observed, while BmK AS-1 could significantly increase the pain threshold to 150% at the dose of 1.2 μg. Naloxone could not reverse the central analgesic effect of BmK AS-1.

关 键 词：东亚钳蝎毒素 脊髓 注射 多肽 皮肤痛觉 大鼠 

分 类 号：R96[医药卫生—药理学]