人牙髓组织中骨形成蛋白-3基因表达的原位分子杂交研究  

Expressing of BMP3 mRNA in human pulp tissue

作　　者：王忠东[1] 文玲英[1] 金岩[1] 顾广玉[2] 肖明振[1] 司晓辉[1] 

机构地区：[1]第四军医大学口腔医学院,710032 [2]第二军医大学附属长海医院病理科,200433

出　　处：《实用口腔医学杂志》2000年第4期275-278,共4页Journal of Practical Stomatology

摘　　要：目的：研究ＢＭＰ３基因在人正常、龋病及炎症状态下牙髓组织中的表达。方法：利用地高辛甙元标记的ＢＭＰ３ ｃＤＮＡ探针对人牙髓组织进行原位分子杂交。结果：正常人牙髓组织的成牙本质细胞有弱阳性杂交信号出现。在有修复性牙本质形成的深龋牙髓组织和有脓肿形成的炎症牙髓组织中，在修复性牙本质下方的成牙本质细胞有丰富的阳性杂交信号出现，还可同时见到在血管周边有阳性杂交信号的未分化间充质样细胞。结论：成熟的功能性成牙本质细胞在一定程度的龋源性炎症刺激下，其ＢＭＰ３基因激活并表达，对牙髓组织的自身修复过程具有重要意义；其表达的强弱与细胞的分化程度及所处的功能状态相关。To study the expression of BMP3 mRNA in human normal, carious and inflammatory pulp tissues. Methods: In situ hybridization was used with a digoxigenin-labelled BMP3 cDNA probe. Results: Weak BMP3 mRNA positive signal was observed in the odontoblasts of normal pulps; while stronger signal in the odontoblasts under the reparative denim layer in the pulps of deep canes and circumscribed inflammatory tissue;BMP3 expression was also observed in mensenchymal cells. Conclusion: The difference of of BMP3 mRNA expression suggests that BMP3 may take part in the process of pulp repair and might be related to the differentiation and biological state of the cells.

关 键 词：牙髓 骨形成蛋白-3 原位杂交 基因表达 龋齿 

分 类 号：R780.2[医药卫生—口腔医学]