清开灵抗大鼠谷氨酸神经毒性脑损伤的实验研究  

Experimental Study of Protective Effect of Qingkailing on Glutamate Induced Neurotoxic Damage of Brain

作　　者：岳少杰[1] 曾庆善[2] 周建华[2] 陶永光[1] 曾珊[2] 伍赶球[2] 虞佩兰[1] 罗自强[2] 

机构地区：[1]湖南医科大学湘雅医院儿科,长沙410008 [2]湖南医科大学

出　　处：《中国中西医结合杂志》2000年第11期842-845,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助课题!(No .3970 0 1 93)

摘　　要：目的 :观察清开灵对大鼠谷氨酸神经毒性脑损伤的作用 ,以寻找有效的抗谷氨酸神经毒性的中西药物。方法 :采用细胞形态计量、电镜及免疫组织化学方法 ,观察清开灵对谷氨酸所致大鼠脑损伤的影响。结果 :清开灵可对抗谷氨酸所致的大鼠脑组织水、钠含量的增加 ,减轻谷氨酸所致的皮层及海马细胞的形态结构的损伤 ,降低脑组织中c fos阳性细胞率。结论 :清开灵具有抗谷氨酸神经毒性作用 ,其作用机制与抑制谷氨酸所致的cObjective: To observe the effect of Qingkailing (QKL) on glutamate induced damage of brain, in order to seek for effective drugs for antagonizing neurotoxicity of glutamate. Methods: Number and morphology metrology of neuron in cerebral cortex and hippocampus were detected by using MIAS 300 image analysing instrument, electron microscope and immunohistochemical methods. Results: QKL could alleviate the glutamate induced accumulation of water and sodium in the brain tissue, relief the damage of cerebral cells and reduce the percentage of c fos positive cell in brain. Conclusion: QKL could protect brain damage induced by glutamate, which might be related to inhibition of QKL on glutamate caused enhancement of c fos gene expression.

关 键 词：谷氨酸 清开灵 实验研究 神经毒性脑损伤 

分 类 号：R285.5[医药卫生—中药学]