实时荧光定量PCR检测人宫颈癌细胞赖氨酰氧化酶样-4基因的表达  

Expression of human cervical cancer lysyloxidase-like 4 gene detected by real time fluorescent quantitative PCR

作　　者：王秀萍[1] 于祥远[1] 董滨华[1] 毛晓丹[1] 孙蓬明[1] 

机构地区：[1]福建省福州市第一医院妇产科,350009

出　　处：《中国妇幼保健》2013年第22期3653-3655,共3页Maternal and Child Health Care of China

基　　金：福州市科技计划项目〔2010-S-88〕

摘　　要：目的:利用实时荧光定量PCR检测赖氨酰氧化酶样-4(Lysyloxidase-like 4,LOXL4)基因在Hela、ME180、HCC94人宫颈癌细胞中的表达情况。方法:提取3株体外培养宫颈癌细胞株的总RNA,逆转录为cDNA,实时荧光定量PCR扩增,结合内参基因GAPDH及标准曲线比较LOXL4 mRNA在Hela、ME180和HCC94 3株体外培养的人宫颈癌细胞中的表达。结果:LOXL4基因在3株人宫颈癌细胞中均有表达,但表达水平存在统计学差异,以Hela的表达为参照(Ratio=LOXL4/GAPDH=1.00),ME180的表达水平为6.80E-3,HCC94细胞株表达丰度最低为5.62E-4。结论:建立了LOXL4基因在宫颈癌细胞表达的检测平台,为宫颈癌早期诊断及预测预后提供可借鉴的观察指标。Objective： To detect the expressions of lysyloxidase-like 4（LOXL4） gene in human cervical cancer HeLa cells,ME180 cells and HCC94 cells by real-time fluorescent quantitative PCR.Methods： The total RNA of three cervical cancer cell lines cultured in vitro was extracted,then it was reverse-transcribed into cDNA and amplified by real-time fluorescent quantitative PCR.The reference gene GAPDH and standard curve were used to compare the expressions of LOXL4 mRNA in human cervical cancer HeLa cells,ME180 cells and HCC94 cells.Results： LOXL4 gene expressed in the three human cervical cancer cell lines,but there was statistically significant difference in the expression level;taking the expression in HeLa cells as reference index（ratio = LOXL4 / GAPDH = 1.00）,the expression level in ME180 cells was 6.80E-3,the lowest expression level in HCC94 cells was 5.62E-4.Conclusion： The detection platform of LOXL4 gene expression in cervical cancer cells is established,which provides a observation index for early diagnosis and predicting prognosis of patients with cervical cancer.

关 键 词：宫颈癌 LOXL4基因 细胞株 荧光定量PCR 

分 类 号：R737.33[医药卫生—肿瘤]