左旋延胡索乙素对大鼠吗啡条件性位置偏爱效应及纹状体多巴胺系统的影响  

Effects of l-tetrahydropalmatine on conditioned place preference and expression of dopamine in the striaturn of morphine dependent rats

作　　者：白威峰[1] 杨培润[1] 余守洋[1] 涂平[1] 吴明松[1] 钱刚[1] 罗素元[1] 

机构地区：[1]遵义医学院细胞生物学与遗传学教研室,遵义563003

出　　处：《中华行为医学与脑科学杂志》2013年第7期591-593,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（30860373）

摘　　要：目的观察左旋延胡索乙素（1-THP）对吗啡诱导大鼠条件性位置偏爱（CPP）效应、纹状体多巴胺递质含量和D2受体表达的影响。方法大鼠吗啡颈背部皮下注射CPP训练10d，剂量从每日10mg／kg逐日递增至100mg／kg，末次训练后48h行CPP检测确认模型建立成功，1-THP分别3．76，1．88，0．94mg／kg灌胃治疗6d再次CPP检测后，取脑纹状体采用高效液相色谱法和免疫组化分别检测其多巴胺递质含量和D2受体的表达。结果1-THP3．76mg／kg和1．88mg／kg治疗组大鼠在伴药箱（白箱）中的停留时间[（354±58）S、（373±79）S]比生理盐水治疗组[（523±55）s]减少；同时其纹状体中多巴胺递质含量（5．49±1．95、6．11±1．05）比生理盐水治疗组（7．37±0．93）降低、D2受体平均吸光度值（0．08±0．02、0．07±0．03）较生理盐水治疗组（0．06±0．02）上调，差异均具有统计学意义（P〈0．01或P〈0．05）。结论1—THP可加速大鼠吗啡CPP效应消退，可能与逆转吗啡CPP模型大鼠纹状体中多巴胺递质含量和D2受体表达有关。Objective To observe effects of conditioned place preference （CPP） of morphine dependent rats, variation of dopamine neurotransmitter and its receptor 2 of the striatum in rats suffered from 1-tetrahydropalmatine（1-THP）. Methods The CPP model was established by morphine injection in rats with a increasing dose for 10 days,with the initial dose of 10 mg . kg^-1 and the final dose of 100 mg .kg^-1 ,10 mg. kg^-1 was increased each day,thus 100 mg . kg^-1 was injected by day 10. Treatments with administration of 1-THP（ 3.76,1.88 and 0.94 mg/kg） were performed respectively for 6 days,and the effects of CPP for psychological dependence in these rats were observed. Striatum samples were taken out and their variable contents of dopamine neurotransmitter and its receptor 2 in striatum were detected by high performance liquid chromatography and immunohistochemisty, re- spectively. Results Compared with the NS treatment group, the time of animals treated with 1-THP （3.76 and 1.88 mg/kg） staying in drug-paired compartment were reduced （ 354 ＋ 58,373 _＋ 79） （P 〈 0.01 ） ; the raised of variation contents of dopamine neurotransmitter were reduced in striatum from those rats （ 5.49 ±1.95,6.11± 1.05 ）, while the expression level of dopamine receptor 2 was increased （0.08±0.02,0.07 ± 0.03 ） （P 〈 0.01 or P 〈 0.05）. Conclusion Reversing dopamine neurotransmitter and its receptor 2 in striatum of morphine dependent rats may be one of the possible mechanisms that 1-THP effectively inhibit the effects of morphine CPP.

关 键 词：左旋延胡索乙素 吗啡 精神依赖 多巴胺系统 

分 类 号：R74[医药卫生—神经病学与精神病学]