多胺类似物双乙基去甲精胺联合阿司匹林高效杀伤宫颈癌Caski细胞的研究  

Effects of polyamine analogue BENS combined with aspirin on the apoptosis of cervical cancer Caski cells

作　　者：杨建林[1] 韩钰[1] 王艳林[1] 张伟[1] 

机构地区：[1]三峡大学分子生物学研究所,湖北省宜昌市443002

出　　处：《实用医学杂志》2013年第15期2443-2447,共5页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:30772590)

摘　　要：目的:研究多胺类似物双乙基去甲精胺(BENS)与阿司匹林联用对宫颈癌Caski细胞的生长影响以及可能的细胞学机制。方法:多胺类似物BENS联用阿司匹林孵育体外培养的人宫颈癌Caski细胞,采用四甲基偶氮唑蓝法测定细胞存活率,DNA Ladder以及流式细胞术观察各组细胞周期和凋亡率变化,Western blot检测Bcl-2、Bax和cyt-C凋亡相关基因的表达情况,化学发光法测定精胺氧化酶(SMO)活性。结果:BENS与阿司匹林联用能显著降低单用BENS的细胞存活率(P<0.05),同时低剂量阿司匹林就能显著增强BENS诱导细胞凋亡作用(P<0.01)。联合用药组与单独用药组相比较,细胞G0/G1期比例明显升高,S期和G2/M期比例下降;诱导细胞凋亡发生;胞浆中Bcl-2表达量减少,Bax表达增加,cyt-C由线粒体释放。且SMO活性无明显改变。结论:联用低剂量阿司匹林能有效增强BENS诱导人宫颈癌Caski细胞凋亡的作用,但与多胺代谢过程无关。Objective To study the synergistic effects of polyamine analogue （bis-ethylnorspermine, BENS） combined with aspirin on the apoptosis of cervical cancer Caski cell lines. Methods Human cervical cancer Caski cell lines cultivated in vitro were administered with BENS combined with aspirin, and then MTY method was used to assay the proliferation of Caski cells. Sub-G1/flow cytometry and DNA fragmentation assays were used to determine the apoptosis. The expressions of apoptosis-related proteins, such as Bcl-2, Bax and cyt-C were measured by Western blot, and the enzymatic activity of spermine oxidase （SMO） was determined by chemical analysis. Results The combination of BENS and aspirin could increase the apoptosis rate of Caski cells markedly （P 〈 0.05）, and low dose of aspirin could markedly increased the apoptosis-inducing ability of BENS on Caski ceils （P 〈 0.01）. Under the combination of BENS and aspirin, the ratio of cells in G0/G1 phase was increased, whilst those in S and G2/M phase were decreased; the expression of Bcl-2 was decreased, that of Bax was increased, and cyt-C was released from mitochondria. The activity of SMO was not changed significantly. Conclusion Aspirin could markedly increase the apoptosis-inducing ability of BENS on Caski cells, which was not related to the activity of SMO.

关 键 词：宫颈肿瘤 癌多胺类似物 BENS 阿司匹林 CASKI细胞 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤]