响应面分析法优化当归粗多糖提取工艺  

Optimization of Extraction Process of Angelica sinensis Polysaccharide Using Response Surface Methodology

作　　者：纪鹏[1] 张蔓 孙红国[1] 宋志学[1] 华永丽[1] 魏彦明[1] 

机构地区：[1]甘肃农业大学动物医学院,兰州730070

出　　处：《天然产物研究与开发》2013年第7期976-981,888,共7页Natural Product Research and Development

基　　金：国家自然科学基金项目(30972210;31272600);甘肃农业大学校创新基金(GAU-C*1102)

摘　　要：选取岷县当归药材为原料,采用水提醇沉法进行多糖提取,以当归粗多糖得率为指标,探讨加水量、回流提取时间、水提液的浓缩比、醇沉后所达含醇比例对当归多糖得率的影响。在单因素分析的基础上,采用响应面分析法(RSM)确定了当归粗多糖最佳提取条件为:加水量837.6 mL/100 g,浓缩后溶液体积为228.12 mL,最终含乙醇浓度为65.80%,回流提取时间2 h,在此条件下预测当归粗多糖得率理论的最佳值为10.44%,实际验证值为10.40%,两者相符,说明RSM法分析的可靠性。In this paper, Angelica medicinal materials in Mincounty was used as the testing material. Its polysaccharide component was extracted by water and precipitated by ethanol. Angelica polysaccharide yield was set as the evaluating index to optimize the extraction process. Four factors, namelyadded watervolume, reflux extraction time, concentration ra- tio of water extracts, ethanol concentration after water extraction and alcoholic precipitation, was optimized by single-fac- tor experiment and response surface methodology （RSM）. The results indicated that the optimum extraction conditions were as follows ：837.6 mL/100 gas addedwater volume ;2has reflux extraction time ;228.12 mLas solution volume after solution concentration ;65.80% as the ethanol concentration after water extraction and alcoholic precipitation. Under the optimized conditions,the predicted value was 10.44%, the actual yield of Angelica sinensis polysaccharide was deter- mined to be 10.40% which was matched with the predicted value. RSM was proved to be reliable.

关 键 词：当归 粗多糖 单因素分析 响应面分析 

分 类 号：R283.1[医药卫生—中药学]