短波紫外对铜绿微囊藻的灭活与微囊藻毒素的降解  

Inactivation of Microcystis aeruginosa and Degradation of Microcystin-LR Using Short Wavelength Ultraviolet

作　　者：欧桦瑟[1] 韦朝海[1] 高乃云[2] 吴海珍[1] 吴超飞[1] 任源[1] 胡芸[1] 

机构地区：[1]华南理工大学环境与能源学院,广东广州510006 [2]同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室,上海200092

出　　处：《中国给水排水》2013年第15期39-42,48,共5页China Water & Wastewater

基　　金：中国博士后科学基金资助项目(2012M521603);国家自然科学基金资助项目(U1201234)

摘　　要：采用短波紫外(UVC)对铜绿微囊藻悬浊液进行预处理,从光合活性、细胞生物量以及藻毒素浓度等方面对UVC的杀藻效果和安全性进行评估。不同剂量的UVC处理对光合量子产率(Φe)的影响显著,对胞内和胞外MC-LR有显著降解效果,照射剂量为350 mJ/cm2时去除率分别达到50%和87%,但对活体Chl-a浓度和细胞完整性的影响不明显。培养试验表明,140 mJ/cm2的UVC照射能有效抑制细胞生长,Φe、细胞密度和Chl-a分别从0.37、3.9×106个/mL、721μg/L缓慢升高到第6天的0.45、5.1×106个/mL和1 430μg/L;胞内和胞外MC-LR分别从82、8μg/L增加到第6天的110、20μg/L,以上指标均显著低于健康细胞样品的培养试验结果。700 mJ/cm2的UVC照射样品培养2 d时,Φe、细胞密度和Chl-a分别降低到0、0.4×106个/mL和282μg/L,细胞群体基本消亡;但胞外MC-LR从零增加到第6天时的32μg/L。综上所述,UVC能破坏藻细胞的光合系统,同时有效降解MC-LR,但过量UVC照射将导致细胞大量死亡,释放并升高胞外MCLR水平,具有潜在的威胁。因此,针对特定浓度含藻原水需要优选适宜的UVC照射剂量。Short wavelength ultraviolet （UVC） irradiation was used to treat Microcystis aeruginosa suspension. The photosynthesis activity, cell biomass and microcystin concentration were used to assess the inactivation efficiency and safety. UVC irradiation induced significant decrease of light quantum yield （фe）, while the removal of intracellular and extracellular microcystin-LR （MC-LR） reached 50% and 87% after 350 mJ/em2 UVC irradiation. However, the change of in vivo chlorophyll-a （Chl-a） concen- tration and cell integrity was small. In cultivation experiment, the фe, cell density and Chl-a of 140 mJ/em2 treated samples increased from 0.37, 3.9× 106 cell/mL and 721 μg/L at 0 d to 0.45, 5.1× 106 cell/mL and 1 430 μg/L at 6 d, indicating a suppression of cell growth. The intraeellular and extra-cellular MC-LR increased from 82 μg/L and 8 Ixg/L at 0 d to 110 μg/L and 20 μg/L at 6 d, which were lower than those in heath samples. The фe, cell density and Chl-a of 700 mJ/em2 treated samples de- creased to 0, 0.4 x 106 cell/mL and 282 μg/L at 2 d, indicating significant death of cells. However, the concentrations of extracellular MC-LR increased from 0 at 0 d to 32μg/L at 6 d. UVC induced critical impairment of photosynthesis, while MC-LR was degraded. However, excessive UVC irradiation induced significant death of cells, resulting in MC-LR release. The UVC dosage should be carefully chosen ac- cording to the biomass in algae-containing suspension.

关 键 词：紫外 铜绿微囊藻 藻毒素 灭活 降解 

分 类 号：X173[环境科学与工程—环境科学]