液质联用代谢组学研究多氯联苯和二噁英对大鼠毒性作用  

Study on the toxicity of polychlorinated biphenyls and dioxin on rats by liquid chromatography-mass spectrometry based metabonomics

作　　者：陈蓉[1] 王以美[2] 汪江山[3] 卢春凤[2] 张凤霞[3] 胡春秀[3] 彭双清[2] 许国旺[3] 

机构地区：[1]中国药科大学理学院分析化学教研室,南京211198 [2]军事医学科学院疾病预防控制所毒理学评价研究中心,北京100071 [3]中国科学院大连化学物理研究所,中国科学院分离分析化学重点实验室,大连116023

出　　处：《环境化学》2013年第7期1226-1235,共10页Environmental Chemistry

基　　金：国家自然科学基金(21175132,20835006);国家重点基础研究发展计划(973计划)课题(2011CB503803);国家科技支撑计划课题(2006BAK02A02)资助

摘　　要：采用基于液质联用(LC-MS)的代谢组学方法研究多氯联苯(PCBs)和2,3,7,8-四氯二苯并-p-二噁英(TCDD)及联合染毒对大鼠生化代谢的影响.对雄性Sprague-Dawley大鼠连续3 d分别灌胃TCDD(10μg.kg-1)、PCBs(Aroclor 1254,10 mg.kg-1)及其混合溶液(10μg.kg-1 TCDD和10 mg.kg-1 Aroclor1254),采用液质联用法在尿液和血浆样本中分别检测出749个和343个色谱峰.PCBs、TCDD及其联合染毒后引起大鼠生化代谢的显著变化.多变量分析结果表明,毒性的大小是:联合染毒>TCDD>PCBs.采用标准物对照、准确质量数、多级质谱碎片离子图和数据库检索的方法,分别在尿液和血浆样本中鉴定出8个和20个生物标记物,表明PCBs和TCDD能导致免疫系统、肝脏和神经系统障碍、干扰脂代谢.This study was to investigate the toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin （TCDD）, PCBs andtheir joint action on rats by the approach of urinal and plasma metabolic profiling. In a consecutive 3-days study with male Sprague-Dawley rats, TCDD （101μg·kg-1）, PCBs （Aroclor 1254, 10 mg·kg-1）, and their combination （10μg·kg-1 TCDD and 10 mg＇kg-a Aroclor 1254） were repeatedly administered by gavage. Responses of rats to these pollutants in the early stage were assessed by metabolic changes in urine and blood. There were 749 and 343 chromatographic peaks detected in urine and plasma samples respectively. Based on multivariate analysis, toxic effects of PCBs, TCDD and their combination were revealed and the significantly changed metabolites were identified. These effects were more remarkable in the combined-exposure group. Identified biomarkers indicate that exposure to TCDD, PCBs or their combination induced disorder of immune system, as well as and nerve system disorder and significant metabolic disturbance in lipid metabolism.

关 键 词：多氯联苯 二噁英 代谢组学 液质联用 

分 类 号：X174[环境科学与工程—环境科学]