复方硫磺洗剂微生物限度检查方法的建立  

Establishment of Method for Microbial Limit Test of Compound Sulfur Lotion

作　　者：王海华 

机构地区：[1]南宁食品药品检验所,南宁530001

出　　处：《中国药房》2013年第29期2770-2772,共3页China Pharmacy

摘　　要：目的:建立复方硫磺洗剂微生物限度检查方法。方法:采用平皿法、培养基稀释法及薄膜过滤法并在冲洗液中加入增溶剂聚山梨酯80,通过比较回收率来确定适宜的检查方法。结果:采用平皿法、培养基稀释法、薄膜过滤法(冲洗液中不含增溶剂),枯草芽孢杆菌的回收率均低于70%;采用薄膜过滤法(在冲洗液中加入增溶剂),回收率达85%以上。控制菌采用培养基稀释法可检出试验菌。结论:建立的方法可考虑作为复方硫磺洗剂的常规微生物限度检查方法。OBJECTIVE：To establish a method for microbial limit test of Compound sulfur lotion.METHODS：The plating method,media dilution method and membrane filtration method were used and the solubilizer polysorbate-80 was added into the solution.The optimal test method was obtained by comparing the recovery rates of test organisms.RESULTS：By plating method,media dilution method and membrane filtration method（solubilizer wasn＇t added into the solution）,the recovery rate of Bacillus subtilis was less than 70%;however,by membrane filtration method（the solubilizer was added into the solution）,the recovery rate was more than 85%.The test organisms were successfully detected in the control bacteria test by media dilution method.CONCLUSIONS：The method can be considered for microbial limit test of Compound sulfur lotion.

关 键 词：复方硫磺洗剂 微生物限度检查 薄膜过滤法 增溶剂 

分 类 号：R927.11[医药卫生—药学] R986