p27^(kip1)的亚细胞定位对卵巢癌细胞周期的作用研究  

Effect of the subcellular localization of p27^(kip1) in different cell cycle of ovarian carcinoma

作　　者：刘沅林[1] 花敏慧[1] 张玉泉[1] 

机构地区：[1]南通大学附属医院妇产科,南通226001

出　　处：《南通大学学报（医学版）》2013年第4期270-273,共4页Journal of Nantong University(Medical sciences)

基　　金：南通市应用研究计划资助项目(K2010046)

摘　　要：目的 :研究卵巢癌中抑癌基因p27kip1蛋白在不同细胞周期中的亚细胞定位及表达形式,分析其对卵巢癌细胞周期调控的作用机制。方法:以人卵巢癌细胞株SKOV3为研究对象,用血清饥饿法同步细胞周期,采用免疫荧光法测定不同细胞周期中p27kip1、p-Ser10p27kip1蛋白的表达定位情况,并用Western Blot法检测p27kip1、p-Ser10p27kip1在不同细胞生长周期的表达。结果:在G0/G1期卵巢癌细胞中,p27kip1定位表达于细胞核,发挥抑制细胞周期的活性的作用。在S期细胞中,p27kip1以p-Ser10p27kip1的形式存在于胞质中。p27kip1的表达水平随着血清释放的时间延长而逐渐减少。p-Ser10p27kip1表达水平随着血清释放的时间延长而逐渐增加。结论:p27kip1定位于卵巢癌细胞核,抑制细胞周期活化,并以磷酸化的形态转移出核,丧失抑癌活性。Objective： To explore the subcellular localization and the expression of p27^kip1 protein in different cell cycle of ovarian carcinoma.Methods： We take the human ovarian cancer cell line SKOV3 as the object of the research.With the serum starvation method we synchronize the cell cycle.Then we detected the localization of p27^kip1 and p-Ser10p27^kip1 protein by immunofluorescence assay.At last we use immunoblotting method to check the expression of p27^kip1 and p-Ser10p27^kip1 in different cell cycles.Results： In the G0/G1 phase cells,p27^kip1 protein localized in the cell nucleus and inhibited the activiation of cell cycles,while in the S phase cells,it was phosphorylated in cytoplasm as p-Ser10p27^kip1.The expression level of p27^kip1 decreased with the time of serum releasing,which opposite to the p-Ser10p27^kip1.Conclusion： The p27^kip1 protein localized in the cell nucleus and inhibited the activiation of cell cycles.It was transported outside of the cell nucleus with the phosphorylated form and lost its function.

关 键 词：卵巢癌 细胞周期 P27KIP1 p-Ser10p27kip1 

分 类 号：R737.9[医药卫生—肿瘤]