小檗碱对高脂饮食诱导的小鼠慢性肾脏损伤和巨噬细胞的影响  

Effect of berberine on high-fat diet induced chronic kidney injury and macrophages in mice

作　　者：赵晔[1] 陈文[2] 朱一帆[2] 刘林[1] 曹长春[1] 

机构地区：[1]南京医科大学附属南京医院肾内科,江苏南京210006 [2]南京医科大学附属南京医院胸心外科,江苏南京210006

出　　处：《南京医科大学学报（自然科学版）》2013年第7期937-942,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：中华医学会临床医学科研专项资金(09010290184);江苏省医学重点人才(RC2011018)

摘　　要：目的:观察小檗碱对高脂饮食诱导的小鼠慢性肾脏损伤的影响及肾脏内巨噬细胞的变化。方法:将30只6周龄C57BL/6小鼠随机分为正常对照组、高脂饮食组、小檗碱治疗组,每组10只,检测总胆固醇、甘油三酯、血肌酐、尿素氮、胱抑素C(Cys C)、24 h尿量和尿白蛋白,HE染色观察肾脏形态病理学变化。Western blot检测白细胞介素(IL)-1β和诱导型一氧化氮合酶(iNOS)的表达水平。免疫荧光染色检测巨噬细胞标记物F4/80的表达变化。免疫组化染色观察M1型巨噬细胞标记物iNOS的表达情况。结果:高脂饮食组小鼠血脂、Cys C、24 h尿量和尿白蛋白水平显著升高(P<0.05);IL-1β、F4/80和iNOS在肾脏表达显著增高(P<0.05)。小檗碱治疗组小鼠血脂、Cys C、24 h尿量和尿白蛋白水平有所降低;IL-1β、F4/80和iNOS的表达均较高脂饮食组减少(P<0.05)。结论:小檗碱可以减轻高脂饮食诱导的小鼠慢性肾脏损伤,这种保护作用是通过减少肾脏组织浸润的巨噬细胞,尤其是减少M1型巨噬细胞的浸润而发挥的。Objective：To observe the effect of berberine on high-fat diet induced mice chronic kidney injury and the macrophages in the kidney.Methods：A total of 30 C57BL / 6 mice were randomly divided into the normal control group,high-fat diet group and berberine treatment group.At the end of experiment,the mice were sacrificed.Blood,urine and kidney tissue were collected.Blood was used for detecting total cholesterol,triglyceride,serum creatinine,urea nitrogen,cystamin C.Urine was used for detecting 24 h urinary albumin.Pathological changes were observed by light microscope by HE staining.The expression of IL-1β and iNOS were detected by Western blot.The expressions of F4 / 80 and iNOS were determined by immnofluorescence staining and immunohistochemistry staining,respectively.Results：Compared with the normal control group,total cholesterol,triglyceride,cystamine C,urine volume and 24 h urinary albumin were significantly higher in the high-fat diet group（P 0.05）.The expressions of IL-1β, F4 / 80 and iNOS increased significantly in the high-fat diet group（P 0.05）.Compared with the high-fat diet group,the berberine treatment group showed less aggravated kidney pathological changes.The expressions of IL-1β,F4 / 80 and iNOS in the berberine treatment group were decreased than in the high-fat diet group（P 0.05）.Conclusion：Berberine may decrease the macrophages, especially the M1 macrophages and then intervene the chronic kidney injury induced by high-fat diet.

关 键 词：小檗碱 高脂饮食 巨噬细胞 慢性肾脏损伤 

分 类 号：R692[医药卫生—泌尿科学]