TNF-α及IL-1β与多形核白细胞在牙周炎症组织中浸润的关系  

The role of IL-1β and TNF-α in hyper-reactivity of neutrophils in rapidly progressive periodontitis patients

作　　者：刘荣坤[1] 曹采方[1] 孟焕新[1] 高岩[2] 

机构地区：[1]北京医科大学口腔医学院牙周科,100081 [2]北京医科大学口腔医学院病理科,100081

出　　处：《中华口腔医学杂志》2000年第5期327-329,I025,共3页Chinese Journal of Stomatology

基　　金：高等院校博士学科点专项科研基金!资助项目(95 0 10 2 0 )

摘　　要：目的　探讨快速进展性牙周炎 (rapidlyprogressiveperiodontitis,RPP)患者局部牙周组织中多形核白细胞 (polymorphonuclearneutrophil,PMN)过度浸润及激活与肿瘤坏死因子α(tumornecrosisfactor α ,TNF α)和白细胞介素 1β(interleukin 1β ,IL 1β)的关系。方法　采用夹心ELISA法检测 2 2例RPP患者共 41个位点的龈沟液TNF α水平 ,并采用酶促反应法检测同一份龈沟液中的PMN特异酶———弹性蛋白酶 (elastase,EA)的活性 ,通过Spearman相关检验分析TNF α水平与EA活性的相关性。另取 10例RPP患者的牙龈组织 ,采用免疫组化法检测TNF α和IL 1β蛋白的表达 ,通过HE染色观察PMN浸润。结果　龈沟液中的TNF α水平 [(3.2 3± 1.81)ng/位点 ]与EA活性 [(0 .5 4± 0 .37)Abs/位点 ]呈显著正相关 (r=0 .44 ,P <0 .0 5 ) ;牙龈组织中的TNF α和IL 1β蛋白表达与PMN浸润密切相关 ;有大量PMN浸润的组织 ,炎症浸润广泛 ,袋上皮的增生与溃疡显著。结论　与TNF α和IL 1β等细胞因子相关的PMN的过度浸润与激活促进了RPP的进展。Objective Neutrophils (PMNs) from rapidly progressive periodontitis (RPP) was found to generate abnormally high levels of oxygen radicals. Elastase activity in gingival crevicular fluid (GCF) from RPP was also found much higher. It suggested that PMNs in some RPP patients are hyper-reactive. The purpose of this study was to investigate the mechanism of PMN hyper-reactivity by surveying the correlation of TNF-α level with elastase activity in GCF and by evaluating the association between PMN infiltration and the expression of IL-1β and TNF-α in gingival tissues from RPP patients. Methods 41 GCF samples from 22 RPP patients and 34 GCF samples from 11 healthy controls were collected. The total amount of TNF-α in GCF was detected using ELISA. The elastase activity was measured with a low molecular weight substrate (S2484) specific for granulocyte. The correlation of TNF-α level with elastase activity in a GCF sample was analyzed with Spearman correlation. 20 gingival specimens were obtained respectively from 10 RPP patients and 5 periodontally healthy controls. The expression of IL-1β and TNF-α was detected with immunohistochemistry. The distribution of PMN was observed with hematoxylin and eosin staining. Results Total amount of TNF-α in GCF was positively correlated with elastase activity ( r =0.44, P < 0.05). The IL-1β- and TNF-α-positive cells in gingiva were superimposed in areas where PMNs infiltration predominant. Conclusion The hyper-reactivity of PMN in RPP patients was related to locally produced IL-1β and TNF-α.

关 键 词：牙周炎 中性白细胞 肿瘤坏死因子Α IL-1Β 

分 类 号：R781.4[医药卫生—口腔医学]