大鼠舌癌变过程中核维甲酸受体β mRNA的表达变化  

The expression of RAR β mRNA in rat tongue carcinogenesis

作　　者：刘兴坤[1] 何荣根[1] 陈万涛[1] 周曾同[1] 周晓健[1] 

机构地区：[1]上海第二医科大学口腔医学院口腔颌面外科,200011

出　　处：《中华口腔医学杂志》2000年第5期343-345,I026,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金!资助项目 ( 3 9670 787)

摘　　要：目的　探讨口腔癌变过程中核维甲酸受体β(retinoicacidreceptorβ,RARβ)mRNA表达与上皮细胞分化异常的相关性。方法　原位杂交检测 6 9例 4 硝基喹啉 1 氧化物 (4NQO)口服诱发大鼠舌癌变各期组织RARβmRNA表达。 结果　正常粘膜、单纯性增生、轻中度异常增生、重度异常增生、原位癌、鳞癌的RARβmRNA阳性率分别为 10 0 0 %、87 5 %、75 0 %、72 2 %、45 5 %和 18 8%。　 结论RARβmRNA下调表达与口腔癌变细胞分化异常密切相关 ,可能是口腔癌变的重要分子机制。Objective To elucidate the relationship between the expression of nuclear retinoic acid receptorβ (RARβ)mRNA in oral carcinogenesis and squamous cell aberrant differentiation Methods A total of 69 rat tongue carcinogenesis specimens induced by 4-nitroquinoline-1-oxide (4NQO) were detected for RARβ mRNA by in situ hybridization Results With the progress of tongue carcinogenesis and epithelium cell disdifferentiation, the expression of RARβ mRNA was down-regulated The positive rate of RARβ mRNA in normal, hyperplasia, mild-moderate dysplasia, severe dysplasia, in situ carcinoma and squamous cell carcinoma was 100 0%, 87 5%, 75 0%, 72 2%, 45 5% and 18 8%, respectively Conclusions The down-regulation of RARβ mRNA may be associated with the epithelium cell aberrant differentiation and may be an important molecular mechanism of oral carcinogenesis

关 键 词：动物实验 舌癌 核维甲酸受体β MRNA 基因表达 

分 类 号：R739.8[医药卫生—肿瘤]