四唑染色法测定厚朴种子生活力的条件研究  

Study on the Determination Conditions of Seed Viability of Magnolia officinalis Rehd. et Wils. by TTC Method

作　　者：石磊[1] 徐广灿[1] 杨红兵[1] 

机构地区：[1]湖北中医药大学药学院,湖北武汉430061

出　　处：《安徽农业科学》2013年第13期5706-5706,5730,共2页Journal of Anhui Agricultural Sciences

基　　金：湖北省教育厅科研重点项目(项目编号D20121607)

摘　　要：[目的]探讨厚朴种子生活力的快速测定方法。[方法]采用四唑染色法(TTC法),设置不同的TTC浓度、染色温度和时间,研究厚朴种子生活力测定的适宜条件。[结果]TTC法测定厚朴种子生活力的适宜条件为:采用浓度0.2%的TTC溶液,在55℃的水浴中染色50 min。[结论]该法可作为厚朴种子生活力测定的有效方法。[ Objective] To study on rapid determination method of seed viability of Magnolia officinalis Rehd. et Wils. [ Method] Using TTC method and setting different TTC concentration, dyeing temperature and dyeing time, the suitable conditions of Magnolia officinalis seed viabil- ity determination were studied. [ Result] The optimal determination conditions for the viability of Magnolia officinalis seed is that staining for 50min in 55 ℃ water bath with 0.5% Trc solution. [ Conclusion] This is an effective method to be used to determine Magnolia officinalis seed viability.

关 键 词：厚朴种子 TTC法 种子生活力 

分 类 号：S567[农业科学—中草药栽培]