鼠视网膜感光细胞移植术后超微结构观察  

An ultrastructural observation on rat retina after photoreceptor cell implantation

作　　者：王薇[1] 马美云[1] 张惠蓉[1] 白芳 

机构地区：[1]北京医科大学第三医院眼科,100083

出　　处：《中华眼科杂志》2000年第5期375-377,I024,共4页Chinese Journal of Ophthalmology

基　　金：国家自然科学基金资助项目!(39870 847)

摘　　要：目的　以遗传性视细胞变性动物RCS鼠为受体 ,Wistar鼠为供体 ,观察纯视锥、杆细胞移植术后RCS鼠视网膜外层超微结构的变化。方法　应用视网膜板层切削技术或准分子激光技术制备供体层状视细胞 ;视网膜板层外路移植技术 ,获得视网膜移植动物模型。术后 2周及 4周时处死动物 ,眼球常规切片后于光镜及透射电镜下观察受体视网膜。结果　移植的视细胞大多成层排列于受体鼠的视网膜色素上皮层及内颗粒层之间。重新出现的外网状层内可见较完整的神经突触及突触间连接。结论　纯视细胞移植术后 。Objective To further study the retinal neuronal signal Methods The Wistar/RCS (RCS rats are rats with hereditary photoreceptor degeneration) rats were respectively as donors/acceptors, and the retinal pathway was reconstructed with the technique of pure photoreceptor transplantation The photoreceptor layer of the retina was obtained with the technique of retinal whittle by manual method or excimer laser The specimens were got separately at 2 weeks and 1 month after the transplantation and studied under the light and transmission electron microscopes Results Most transplanted photoreceptors with physical poles were lined up regularly between the retinal pigment epithelium (RPE) and inner nuclear layer It was shown that in the new outer plexiform layer the relatively integral synapse and its interconnection were seen Conclusion The retinal neuronal pathway can be reconstructed by retinal transplantation

关 键 词：视网膜 动物实验 感光细胞移植术 大鼠 

分 类 号：R779.6[医药卫生—眼科]