木瓜发酵液对小鼠四氯化碳诱发肝损伤的防护作用  

Protective Effect of Fermented Chaenomeles speciosa Juice on Acute Hepatic Injury Induced in Mice by Carbon Tetrachloride

作　　者：汤超[1] 汤文凡[2] 黄玉琴[1] 李亚坤[1] 赵超[1] 沈雪莲[1] 韩强[1] 刘朝奇[3] 

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]三峡大学人民医院妇产科,湖北宜昌443000 [3]三峡大学分子生物学研究所,湖北宜昌443002

出　　处：《食品科学》2013年第13期271-274,共4页Food Science

基　　金：三峡大学教学研究项目(J2011013)

摘　　要：目的:建立四氯化碳(CCl4)诱发的急性肝损伤模型,在组织形态学和分子水平观察木瓜发酵液对小鼠肝脏损伤的预防和保护作用。方法:普通昆明小鼠随机分组,CCl4注射昆明种小鼠制备急性肝损伤模型,应用木瓜发酵液进行小鼠灌胃,然后测定各组血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性,HE染色观察肝组织病理变化,RT-PCR法检测肝组织炎性因子TGF-β、IL-1β和TLR4 mRNA水平变化。结果:与正常对照组比较,模型组小鼠血清ALT和AST活性升高(P<0.05),提示肝损伤模型成功。与模型组比较,药物干预后小鼠血清ALT和AST活性显著降低(P<0.05),肝组织TGF-β、TLR4、IL-1βmRNA水平均有所降低。HE染色显示,药物组小鼠肝组织学得到明显改善,尤其是在高剂量组。结论:木瓜发酵液对CCl4诱导的急性肝损伤有一定的防护作用,其可能开发为肝毒性损伤治疗的新药物。Objective： The preventive and protective effect of fermented Chaenomeles speciosa juice against liver injury was studied at the morphological and molecular levels in a mouse model of acute liver injury induced by carbon tetrachloride.Methods： Kunming mice were randomly grouped.Mouse models of acute liver injury were established by intraperitoneal injection of CCl4 in mice.Three different（high,medium and low） concentrations of fermented Chaenomeles speciosa juice were used to treat the mice by intragastrical mode at a dose of 20 mL/（kg.d） body weight once a day for eight consecutive days.Then the mice were killed and blood samples were collected and measured for the activities of alanine aminotransferase（ALT） and aspartate aminotransferase（AST） in serum.Hepatic pathological changes were examined by HE staining method.The mRNA expression of TGF-β,IL-1β and TLR4 in hepatic tissues were analyzed with RT-PCR.Results： The activities of AST and ALT revealed an obvious increase in serum,and TGF-β,TLR4 and IL-1β were overexpressed in the hepatic tissues of mouse models of liver injury.Compared with the model group,the activities of ALT and AST in serum and mRNA expression of TGF-β,IL-1β and TLR4 in the treatment groups were decreased.HE staining indicated that the hepatic pathological injury in the treatment groups was improved in a dose-dependent manner.Conclusion： Fermented Chaenomeles speciosa juice has a protective effect on acute liver injury induced by carbon tetrachloride,and promising potential to be developed into a new drug against liver injury.

关 键 词：急性肝损伤 四氯化碳 木瓜发酵液 免疫保护 

分 类 号：R34[医药卫生—基础医学]