矢车菊素-3-葡萄糖苷抑制KKAy糖尿病小鼠PTEN表达  

Cyanidin 3-Glucoside Ameliorates Insulin Sensitivity through Down-regulation of PTEN in Diabetic KKAy Mice

作　　者：卫晓怡[1] 白晨[1] 崔琳琳[1] 陆红[2] 蒲立柠[1] 

机构地区：[1]上海商学院旅游与食品学院,上海200235 [2]复旦大学生命科学学院,上海200433

出　　处：《食品科学》2013年第13期280-284,共5页Food Science

基　　金：上海商学院启明星项目(QMX2012-11);2012年度上海商学院校级教学团队建设项目;上海市教育委员会科研创新项目(10YZ212)

摘　　要：通过在KKAy小鼠膳食中添加花色苷Cy-3-g(1g/kg膳食)干预12周,分析Cy-3-g对小鼠血糖、血清胰岛素含量的作用,并进一步检测肝脏、肌肉、内脏脂肪组织PTEN mRNA、蛋白以及磷酸化Akt蛋白的表达。结果表明:Cy-3-g膳食干预可以改善KKAy小鼠胰岛素敏感性,抑制肝脏、肌肉、内脏脂肪组织PTEN表达而增强Akt磷酸化,提示富含Cy-3-g的花色苷提取物可能是通过抑制PTEN表达而激活PI3K/Akt通路,从而改善小鼠胰岛素抵抗。Cyanidin-3-O-β-glucoside（Cy-3-g）-rich foods have been reported to ameliorate the onset of insulin resistance,but the underlying mechanism has remained unclear.PTEN（phosphatase and tensin homologue deleted on chromosome 10） plays a negative role in insulin signaling-PI3K（phosphatidylinositol 3-kinase）/Akt（protein kinase B）,and its inhibition improves insulin sensitivity.In order to gain insight into the effect of Cy-3-g anthocyanin on insulin sensitivity and the role of PTEN,KKAy mice were fed AIN-93G diet supplemented with or without Cy-3-g（1 g/kg diet） for 12 weeks.Our results demonstrated that Cy-3-g ameliorated insulin sensitivity,and inhibited the PTEN expression in liver,muscle and viscera adipose tissues following the activation of Akt（Ser473） in mice.These findings provided evidence for the role of PTEN in PI3K/Akt（Ser473） pathway,thus suggesting that the possible mechanism may be the contribution of Cy-3-g to insulin signaling.

关 键 词：矢车菊素-3-葡萄糖苷 胰岛素抵抗 PTEN KKAY小鼠 

分 类 号：TQ936.16[化学工程]