脑卒中后抑郁与脑源性神经营养因子基因Val66Met单核苷酸多态性的相关性研究  

The Correlation between Val66Met Polymorphism in Brain-derived Neurotrophic Factor and Post-stroke Depression

作　　者：卢丽萍[1] 朱英标[1] 成俊英[1] 

机构地区：[1]丽水市人民医院神经科,323000

出　　处：《中国临床神经科学》2013年第4期413-417,共5页Chinese Journal of Clinical Neurosciences

摘　　要：目的探讨脑卒中后抑郁(PSD)与脑源性神经营养因子(BDNF)基因Val66Met单核苷酸多态性(SNP)的相互关系。方法选择86例PSD患者为PSD组,68例脑卒中无PSD患者为无PSD组,另选择46名健康者为对照组。3组研究对象年龄及性别基本匹配。采用酶联免疫吸附法检测BDNF浓度和等位基因特异性聚合酶链反应(A-S PCR)技术检测BDNF基因Val66Met位点SNP的分布。结果 PSD组和无PSD组间BDNF表达差异有统计学意义(t=-2.038,P=0.043);两组间基因型频率(χ2=0.340,P=0.844)和等位基因频率(χ2=0.036,P=0.849)差异均无统计学意义。结论 BDNF表达与PSD发生相关,但BDNF基因Val66Met位点SNP与PSD未发现有相关关系。Aim To evaluate the association of Val66Met single nucleotide polymorphism（SNP） in brain-derived neurotrophic factor（BDNF） gene with post-stroke depression（PSD）.Metholds PSD group（86 PD patients）,non-PSD group（68 stroke patients） and 46 healthy control group took part in this research,the age and sex were matched in the groups.BDNF concentrations was tested by enzyme-linked immunosorbent assay,and Val66Met SNP in BDNF gene was detected by Allele-Specific polymerase chain reaction（A-S PCR） technique.Results No significant differences were demonstrated in Val66Met BDNF genotype（c2=0.340,P=0.844） or allele frequencies（c2=0.036,P=0.849） between PSD patients and the controls.The expression of BDNF in PSD patients was more than that in the controls（t=-2.038,P=0.043）.Conclusion The results indicated that the expression of BDNF was associated with PSD,but Val66Met SNP in BDNF gene was not associated with PSD.

关 键 词：脑卒中后抑郁 脑源性神经营养因子 基因表达 单核苷酸 

分 类 号：R743[医药卫生—神经病学与精神病学] R749.1[医药卫生—临床医学]