胃癌中CAGE、MAGE-A1和MAGE-A3的去甲基化  

DNA hypomethylation of CAGE,MAGE-A1 and MAGE-A3 genes in gastric carcinoma

作　　者：王柏清[1] 刘文天[2] 刘应莉[2] 孙光斌 

机构地区：[1]天津市第五中心医院消化科,天津市300450 [2]天津医科大学总医院消化科,天津市300052

出　　处：《世界华人消化杂志》2013年第20期1987-1991,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨肿瘤相关抗原基因(cancer associated antigen gene,CAGE)、黑色素瘤抗原基因-A1(melanoma antigen gene A1,MAGE-A1)、黑色素瘤抗原基因-A3(melanoma antigen gene A3,MAGE-A3)启动子区去甲基化与胃癌临床特点的关系和意义.方法:应用甲基化特异性PCR(methylation specific polymerase chain reaction,MSP)的方法检测30例胃癌和25例正常胃黏膜标本中CAGE、MAGE-A1和MAGE-A3基因启动子区的去甲基化状态,并分析其与胃癌临床病例参数间的关系.结果:胃癌组CAGE、MAGE-A1和MAGE-A3基因启动子去甲基化阳性率(80.0%、60.0%、46.7%)均明显高于正常胃黏膜组(4.0%、0.0%、8.0%,P<0.05).胃癌组中,CAGE基因启动子区去甲基化与淋巴转移和临床分期有关(P=0.016;P=0.026);MAGE-A1基因启动子区去甲基化与组织分化程度和临床分期有关(P=0.042;P=0.002);MAGE-A3基因启动子区去甲基化与淋巴转移和组织分化程度有关(P=0.034;P=0.026).结论:CAGE、MAGE-A1和MAGE-A3基因启动子区去甲基化的检测可能有助于判断胃癌的组织分化程度、淋巴结转移和临床分期,有可能成为胃癌治疗及判断预后的分子标志物.AIM：To investigate the relationship between the hypomethylation of cancer associated antigen gene（CAGE）,melanoma antigen gene A1（MAGE-A1） and melanoma antigen gene A3（MAGE-A3） promoters and clinicopathological features of gastric carcinoma.METHODS：The methylation status of CAGE,MAGE-A1 and MAGE-A3 promoter CpG islands was detected by MSP in 30 gastric carcinoma samples and 25 normal controls.The relationship between hypomethylation of CAGE,MAGE-A1 and MAGE-A3 promoters and clinicopathological features of gastric carcinoma was analyzed.RESULTS：The rates of hypomethylation of CAGE,MAGE-A1 and MAGE-3 promoters in gastric carcinoma were signif icantly higher than those in normal controls（80.0% vs 4.0%,60.0% vs 0.0%,46.7% vs 8.0%,all P 0.05）.Hypomethylation of CAGE promoter was signif icantly correlated with lymphatic metastasis and TNM stage（P = 0.016,0.026）,hypomethylation of MAGE-A1 promoter was signif icantly correlated with tumor differentiation and TNM stage（P = 0.042,0.002）,and hypomethylation of MAGE-A3 promoter was signif icantly correlated with tumor differentiation and lymphatic metastasis（P = 0.034,0.026）.CONCLUSION：The hypomethylation of CAGE,MAGE-A1 and MAGE-A3 promoter CpG islands may be used as a biomarker to estimate the differentiation,lymphatic metastasis and TNM stage of gastric carcinoma.

关 键 词：肿瘤相关抗原基因 黑色素瘤抗原基因-A1 黑色素瘤抗原基因-A3 DNA低甲基化 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]