EGR-1蛋白在人肺鳞状细胞癌组织中的表达及其临床意义  

Expression of EGR-1 protein in human lung squamous cell carcinoma tissues and its clinical significance

作　　者：冯飞[1] 田辉[1] 岳韦名[1] 李林[1] 李树海[1] 高存[1] 司立博[1] 鲁铭[1] 

机构地区：[1]山东大学齐鲁医院胸外科,山东济南250012

出　　处：《中国肿瘤生物治疗杂志》2013年第4期456-460,共5页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金资助项目(No.30571844)~~

摘　　要：目的:检测早期生长反应基因-1(early growth response gene-1,EGR-1)蛋白在肺鳞状细胞癌组织中的表达,探讨EGR-1蛋白表达与患者临床病理指标及预后的相关性。方法:收集2007年1月至2007年12月山东大学齐鲁医院肺鳞状细胞癌83例患者癌组织和癌旁肺组织标本,采用免疫组化SP法检测癌组织和癌旁肺组织中EGR-1蛋白的表达水平。分析EGR-1蛋白表达与患者临床病理指标之间的关系,Kaplan-Meier法计算患者的5年生存率,Log-rank法检验比较患者的生存差别,Cox回归多因素分析判定独立的预后因素。结果:EGR-1蛋白在肺鳞状细胞癌组织中的表达水平显著低于癌旁肺组织[(4.12±0.35)vs(6.90±4.58),P<0.01];EGR-1蛋白低表达与患者年龄(P=0.912)、性别(P=0.429)及肿瘤细胞分化程度(P=0.289)均无显著相关性,与吸烟史(P=0.025)、肿瘤大小(P=0.013)、淋巴结转移(P=0.003)及TNM分期(P=0.028)显著相关。EGR-1蛋白低表达的患者,其术后5年总生存率显著低于EGR-1蛋白高表达的患者(2.6%vs 15.9%,P=0.04);TNM分期(P=0.020)、EGR-1蛋白低表达(P=0.035)是判定肺鳞状细胞癌患者预后的独立因素。结论:EGR-1蛋白在肺鳞状细胞癌组织中存在低表达,且与肿瘤进展及患者预后密切相关。Objective： To detect the expression level of early growth response gene-1 （EGR-1） protein in human lung squamous cell carcinoma tissues, and to investigate the relationship of EGR-1 protein expression with elinicopathologic fac- tors and prognosis of lung squamous cell carcinoma. Methods： Carcinoma and corresponding paracancerous tissues were obtained from 83 lung squamous cell carcinoma patients （ Qilu Hospital of Shandong University during Jan. 2007 to Dec. 2007）. Immunohistochemical staining （ SP method） was performed to detect the expression level of EGR-1 in carcinoma and paracancerous tissues. X^2 test was used to analyze the relationship between EGR-1 protein expression and patients＇ clinicopathologic factors, Kaplan-Meier method was used to calculate the 5-year survival rate of patients, Log-rank test was used to compare survival differences, and Cox regression analysis was used to determine the independent prognostic factor.Results ： The expression of EGR-1 protein in lung squamous cell carcinoma tissues was significantly lower than that in pa- racancerous tissues （ [ 4.12± 0.35 ] vs [ 6.90 ± 4.58 ], P 〈 0.01 ）. EGR-1 protein low expression was not significantly associated with patient＇ s age （ P = 0. 912） and gender （ P = 0. 429 ）, and the differentiation stage of tumor （ P = 0. 289 ）, but significantly associated with patient＇ s smoking history （ P = 0. 025 ）, tumor size （ P = 0. 013 ）, lymph node metastasis （P = 0. 003 ） and TNM stage of tumor （P=0. 028 ）. The patients with a low expression of EGR-1 protein had a signifi- cantly low overall survival （2.5% vs 15.9%, P =0.04） at 5 years after operation compared with patients with a high ex- pression of EGR-1 protein. TNM stage （P = 0. 020） and low expression of EGR-1 protein （ P = 0. 035 ） were independent prognosticators of patients with lung squamous cell carcinoma. Conclusion： EGR-1 protein is low expressed in lung squa-mous cell carcinoma tissues, and is associated with tumor

关 键 词：EGR-1蛋白 肺鳞状细胞癌 预后 

分 类 号：R734.2[医药卫生—肿瘤] R730.2[医药卫生—临床医学]