Cajal间质细胞在大鼠慢传输便秘模型结肠中的变化  

Alterations of Cajal cells in the colon of slow transit constipation rats

作　　者：李震[1] 郑豪[1] 李国宾[1] 智会[1] 袁维堂[1] 

机构地区：[1]郑州大学第一附属医院肛肠外科,450052

出　　处：《中华胃肠外科杂志》2013年第8期777-779,共3页Chinese Journal of Gastrointestinal Surgery

摘　　要：目的研究Cajal间质细胞（ICC）与慢传输型便秘（STC）的关系。方法用复方地芬诺酯灌胃的方法建立大鼠慢传输便秘模型（STC组），Westernblot法测定STC组与对照组大鼠升结肠和降结肠组织ICC的特异性标志物c—kit变化情况，利用其与对应的内参B—actin灰度值的比值作为各组c—kit蛋白相对含量。结果STC组大鼠日均粪便量为（1．3±0．7）g／100g，比对照组的（1．6±0．9）g／100g明显减少，差异有统计学意义（t=10．798，P〈0．05）。STC组首粒黑粪排出时间为（461．6±150．8）min，较对照组大鼠的（351．3±119．9）min显著延长（t=2．291，P〈0．05）。STC组与对照组升结肠c—kit灰度比平均值分别为0．277±0．077和0．576±0．081（t=10．719，P〈0．05）；降结肠c—kit灰度比平均值分别为0．280±0．075和0．571±0．079（t=10．700，P〈0．05）；c．kit在STC组升结肠和降结肠的表达均下调，差异均有统计学意义。结论ICC在升结肠和降结肠的减少可能对慢传输型便秘的发生与发展有一定作用。Objective To investigate the association of expression of c-kit （marker of interstitial cells of Cajal, ICC） in colon with slow transit constipation （STC） in rats. Methods Slow transit constipation （STC） rat model was induced by intragastrical administration of compound diphenoxylate. Western blotting was used to measure the expression of c-kit in colon of STC rats （model group） and normal rats（control group）. Gray scale ratio of c-kit to β-actin was used as the relative quantity of c- kit. Results Fecal quantity per day of STC group was （1.3±0.7） g/100 g, significantly lower than that in normal rats [ （1.6±0.9） g/100 g, t=10.798, P〈0.05 ]. In model rats, the time of discharge of the first black fecal was （461.6±150.8） rain, significantly longer than that in normal rats[（351.3±l19.9） min, t=2.291, P〈0.05]. Western blotting revealed that the average values of gray scale ratio of c-kit in proximal colon were 0.277±0.077 and 0.576±0.081 （t=10.719, P〈0.05）, in distal colon were 0.280±0.075 and 0.571±0.079 （t=10.700,P〈0.05） in model group and control group respectively. Conclusion Down-regulation of c-kit expression in proximal colon and distal colon is associated to the pathogenesis of slow transit constipation in rats.

关 键 词：慢传输型便秘 CAJAL间质细胞 c—kit蛋白 动物模型 大鼠 

分 类 号：R574.62[医药卫生—消化系统]