IKK-NBD多肽通过调节c-rel对抗局灶脑缺血再灌注大鼠大脑皮质炎症反应  

IKK-NBD peptides exert anti-inflammation effect in rats after focal cerebral cortex ischemia /reperfusion via regulating c-rel

作　　者：李扬[1] 罗勇[1] 秦文熠[1] 蔡艳丽[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科,重庆400016

出　　处：《第三军医大学学报》2013年第16期1721-1725,共5页Journal of Third Military Medical University

基　　金：教育部"高等学校博士学科点专项科研基金"联合资助课题(20095503110001);重庆市卫生局中医药科研项目(2012-2-128)~~

摘　　要：目的探讨局灶脑缺血再灌注大鼠不同时间点大脑皮质炎症反应及IKK-NBD多肽干预对抗炎症反应的机制。方法采用线栓法制备Spragne-Dawley(SD)大鼠局灶脑缺血再灌注模型。将大鼠分为假手术组、模型组及IKK-NBD组,再下设再灌注1 d和7 d 2个时相点。IKK-NBD组通过侧脑室定位定量注入4μL IKK-NBD。对各组进行Zea-Longa神经功能评分,HE染色观察病理变化,Western blot与荧光定量PT-PCR分别检测缺血皮质区c-rel蛋白和IκappaBα(IκBα)mRNA表达,ELISA检测缺血区炎症因子IL-1β和IL-10的含量。结果①再灌注1、7 d时,IKK-NBD对模型大鼠行为学改善明显;②再灌注1、7 d时,模型组病理改变较IKK-NBD组明显;③胞核c-rel蛋白:再灌注1、7 d时,模型组均高于假手术组(P<0.05);再灌注1 d时,IKK-NBD组高于假手术组(P<0.05);再灌注1、7 d时,IKK-NBD组低于模型组(P<0.05,P<0.01);随着时间延长模型组和IKK-NBD组均降低(P<0.01);④IκBαmRNA:再灌注1、7 d时,模型组和IKK-NBD组均高于假手术组(P<0.05),7 d较1 d回落;再灌注1 d时,IKK-NBD组高于模型组(P<0.01);⑤IL-1β:再灌注1、7 d时,模型组与IKK-NBD组均明显高于假手术组(P<0.01),浓度随时间降低;再灌注1 d时,IKK-NBD组较模型组减少(P<0.01);IL-10:再灌注1、7 d时,模型组与IKK-NBD组均明显高于假手术组(P<0.01),浓度随时间降低;再灌注1、7 d时,IKK-NBD组均高于模型组(P<0.01)。结论 IKK-NBD在局灶脑缺血再灌注损伤后早期可以通过上调IκBα限制c-rel入核,下调IL-1β,上调IL-10而减轻再灌注后炎症损伤;而脑缺血再灌注恢复期,少量c-rel可上调IL-10起到抗炎作用。Objective To investigate the cerebral cortex inflammation of SD rats model of focal cerebral ischemia/reperfusion and the underlying mechanism of IKK-NBD peptides＇ anti-inflammation effect. Methods The MCAO/R SD rats were randomly assigned to 3 groups, that is, sham operation group, model group and IKK-NBD group, and each group was further randomly divided into 2 subgroups with reperfusion 1 and 7 d after 2 hours＇ ischemia. IKK-NBD of 4 μL was injected into the right lateral ventricle in 2 h before model infliction. Neurobehavior was evaluated by Zea-Longa score. HE staining was used for pathological observation, Western blotting and quantitative RT-PCR for mRNA and protein expression levels of c-rel and IkappaBet （Is：Bet）, and ELISA for the contents of IL-β and IL-10 in the isehemic cortex. Results IKK- NBD improved the behavioral recovery and reduced edema and necrosis after 1 and 7 d of reperfusion. The protein expression of c-rel in the cell nucleus was significantly higher in the model group than in the sham operation group （P 〈 0.05）, but significantly lower in IKK-NBD group than model group in 1 and 7 d after reperfusion （P 〈 0. 05, P 〈 0. 01 ）, while, in 1 d after reperfusion, it was significantly higher in IKK-NBD group than the sham operation group （P 〈 0. 05 ）. It was continuously decreased in the IKK-NBD group and model group with the elapse of time （P 〈 0. 05 ）. Expression level of Is：Bet mRNA was significantly higher in the model and IKK- NBD groups than the sham operation group in 1 and 7 d after reperfusion （P 〈 0. 05 ）, with that in day 7 lower than in day 1, and it was higher in IKK-NBD group than in model group in 1 d after reperfusion （P 〈0. 01 ）.IL-1β and IL-IO were significantly higher in the model and IKK-NBD groups than the sham operation group in 1 and 7 d after reperfusion （P 〈 0. 01 ）, and continued to reduce with the time, but in the day 1, IL-1β was lower in the IKK-NBD group than in the model group （P 〈0. 01 ）, and IL-

关 键 词：局灶脑缺血再灌注 炎症因子 IKK-NBD多肽 C-REL IΚBΑ 

分 类 号：R364.5[医药卫生—病理学] R743.31[医药卫生—基础医学]