富血小板血浆对根尖乳头干细胞增殖的影响  

Effect of platelet-rich plasma on the proliferation of human apical papilla stem cells

作　　者：刘彩霞[1] 王立[1] 

机构地区：[1]唐山市口腔医院儿童口腔科,河北唐山063000

出　　处：《牙体牙髓牙周病学杂志》2013年第8期489-491,540,共4页Chinese Journal of Conservative Dentistry

基　　金：唐山市科技攻关项目(121302108a)

摘　　要：目的:观察不同浓度富血小板血浆(platelet-rich plasma,PRP)对人根尖乳头干细胞(humanapical papilla stem cells,HAPSCs)增殖的影响。方法:两步离心法制备PRP,并用ELISA法测定其中血小板数以及血小板源性生长因子(PDGF-AB)、转化生长因子(TGF-β1)的浓度;组织块法分离、培养HAPSCs,分别用含5%、10%、20%PRP的DMEM中进行培养(以不含PRP的DMEM作对照),在培养1、2、3、4、5 d各时间点以CCK-8法检测各组HAPSCs的增殖活性。结果:所制备的PRP中血小板数和各生长因子浓度均大于全血;与对照组相比,不同浓度PRP对HAPSCs的增殖均有明显促作用(P<0.05),且随培养时间越来越明显,各实验组4 d时的促进增殖作用均明显强于2 d时(P<0.05);不同浓度PRP相比,以10%组促增殖作用最强,与另两组相比各时间点差异均有统计学意义(P<0.05),而5%与20%组相比无统计学差异(P>0.05)。结论:5%、10%、20%的PRP对HAPSCs的增殖均有明显促进作用,其中以10%组最为显著。AIM ： To evaluate the effects of platelet-rich plasma（PRP） on the proliferation of human apical papilla stem cells（HAPSCs）. METHODS： Human PRP were prepared from the volunteers by two step eentrifugation. PDGF-AB and TGF-I31 were measured by enzyme-linked immunosorbent assay （ELISA）. HAPSCs were exposed to PRP at 5% , 10% and 20% respectively. Cell proliferation was examined by CCK-8 assay after 1,2,3,4 and 5 d culture. RESULTS： The concentrations of PDGF-AB and TGF-131 in PRP were higher than those in the whole blood. The most obvious effects on proliferation were in 10% PRP treated cells. The effect of proliferation on 4 d was greater than that on 2 d. CONCLUSION ： PRP may promote the proliferation of human apical papilla stem cells.

关 键 词：根尖乳头干细胞 富血小板血浆 生长因子 细胞增殖 

分 类 号：R780.2[医药卫生—口腔医学]