两种糖经变异链球菌代谢后酸性产物的定量比较  

Comparison of the acid productivity of Streptococcus mutans exposed to two kinds of sugar

作　　者：黄莉莉[1] 王舒婷[1] 康琼屹[1] 夏文薇[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院·牙体牙髓病科,上海市口腔医学重点实验室,上海200011

出　　处：《牙体牙髓牙周病学杂志》2013年第8期495-498,502,共5页Chinese Journal of Conservative Dentistry

基　　金：上海科学技术委员会项目基金资助(10JC1408800)

摘　　要：目的:通过对高果糖玉米糖浆(high-fructose corn syrup,HFCS)和蔗糖经变异链球菌(S.mu-tans)代谢后主要酸性产物的定量分析,比较两者的致龋性。方法:S.mutans UA159分别接种与含10 g/L HF-CS、含10 g/L蔗糖的两种TSB培养基中,37℃、900 mL/L N2、100 mL/L CO2条件下进行培养。分别于培养后1、4、8、24、48 h各时间点,用高效液相色谱测定两种培养基中乳酸、甲酸、乙酸和琥珀酸的量。结果:S.mutans在两种糖培养基中产生的乳酸最多(与其他各酸相比P<0.05);而其他各酸的产量基本一致(P>0.05);两种培养基中的有机酸产生量在各时间点略有差异,但总趋势相同,即两种糖培养基中各有机酸的产量均随培养时间延长而逐渐增加(P<0.05)。结论:高果糖玉米糖浆和蔗糖经S.mutans代谢后均能产生大量高致龋性的乳酸,说明两者都有较强的致龋性。AIM： To compare the the acid productivity of Streptococcus mutans exposed to high-fructose corn syrup（HFCS） or sucrose . METHODS： Streptococcus mutans were exposed to HFCS at 10 g/L and sucrose at 10 g/L for 1 h, 4 h, 8 h, 24 h and 48 b trspectively. The acid products of formate, acetate, lactic acid and succinic acid were extracted for high-performance liquid chromatography （HPLC） analysis. RESULTS ： Lactic acid was the major metabolite in both glucose medium（P 〈 0.05 ）, the difference of other 3 acid metabolic products between the cultures in the 2 media was not significant（ P 〉 0.05）. The production of acid metabolites at each time points was not equal, but the tendency was the same, acid metabolites increasesed with the cuture time（P 〈 0.05 ）. CONCLUSION ： Streptococcus mutans may produce more acid metabolites when exposed to HFCS or sucrose, indicating HFCS and sucrose have cariogenicity.

关 键 词：高果糖玉米糖浆 变异链球菌 高效液相色谱 

分 类 号：R780.2[医药卫生—口腔医学]