幽门螺杆菌感染对胃癌患者外周血MODCs功能的影响  

Effect of Helicobacter pylori on the function of peripheral blood monocyte-derived dendritic cells in gastric cancer patients

作　　者：李巧珍[1,2] 陈玉强[1,2] 颜江华[3] 丁园[1,2] 王勇军[1,2] 王生育[3] 叶燕华[1,2] 许英艺[1,2] 何蕙香[1,2] 

机构地区：[1]厦门大学附属成功医院 [2]中国人民解放军第一七四医院肿瘤治疗中心,福州市350025 [3]厦门大学医学院抗癌研究中心

出　　处：《中国肿瘤临床》2013年第15期902-906,共5页Chinese Journal of Clinical Oncology

基　　金：南京军区医学科技创新课题(编号:10MA068);福建省自然科学基金资助项目(编号:2010D013);厦门市科技计划创新项目(编号:3502z20104014)资助~~

摘　　要：分析Hp阳性和Hp阴性的胃癌患者外周血单核细胞来源树突状细胞(MODCs)功能的差异性及其临床意义。方法:用尿素14C呼气试验对解放军第一七四医院2011年1月至2012年10月收治的84例胃癌患者进行Hp感染状态鉴定,分别采集Hp阳性和阴性胃癌患者外周血,分离外周血单个核细胞,采用经典方法(rhGM-CSF、rhIL-4联合rhTNF-α)诱导产生DCs,采用流式细胞仪检测DCs表型,采用LDH释放法检测DCs致敏T细胞对胃癌细胞的毒性杀伤作用,采用ELISA方法检测细胞因子IL-12、IFN-γ的分泌水平。结果:两组MoDCs成熟过程形态变化无差异,Hp阳性组MoDCs表面标记分子CD1a、CD80、CD83、CD86和HLA-DR平均表达百分率均高于Hp阴性组,其中CD80、CD83、CD86的表达水平差异有统计学意义,CD1a、HLA-DR差异无统计学意义。Hp阳性组DCs致敏T淋巴细胞对胃癌细胞杀伤率和IL-12、IFN-γ的分泌水平均高于Hp阴性组(P<0.05)。结论:Hp感染状态不影响胃癌患者外周血MoDCs成熟过程形态变化,Hp感染的胃癌患者MoDCs成熟和活化水平更高。Objective： This study aimed to compare and analyze the functional differences between peripheral blood monocyte-derived dendritic cells （DCs） of Helicobacter pylori-positive and H. pylori-negative patients with gastric cancer. Methods： H. pylori infection was detected in 84 patients with gastric cancer in our hospital from January 2011 to October 2012 by the ^14C-urea breath test. DCs were generated from monocytes isolated by an adherent method from the two groups of patients and cultured in the presence of rhlL-4, rhGM-CSF, and rhTNF-cc Furthermore, the expression of surface marker molecules was determined by fluorescence-activated cell sorting analysis. The cytotoxicity of DCs pulsed T cells against gastric carcinoma cell was assessed by the lactate dehydrogenase-releasing assay. The secretion of IL-12 and IFN-γ in the supernatant was determined by enzyme-linked immunosorbent assay. Resuits： No difference was observed in the morphological change of the maturation process. The mean expression of CD 1 a, CD80, CD83, CD86, and HLA-DR molecules in DCs of H. pylori-infected patients was higher than that in DCs of H. pylori-negative group, and the differences were statistically significant except for CDla and HLA-DR. The cytotoxicity activities, IL-12 release, and IFN-γ release in the H. pylori-positive group were significantly higher than those in the H. pylori-negative group （P〈0.05）. Conclusion： H. pylori infection has no effect on the morphological change of the maturation process of monocyte-derived DCs. These data clearly demonstrate that monocyte-derived DCs of H. pylori-infected patients with gastric cancer can induce stronger maturation and activation than those of H. pylori-negative patients.

关 键 词：树突状细胞 幽门螺杆菌 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]