金属硫蛋白-1在小鼠肾脏的表达  

The Expression of Metallothionein-1 in Mouse Kidney

作　　者：田娟[1] 郭敏[1] 

机构地区：[1]辽宁医学院组胚教研室,辽宁锦州121001

出　　处：《天津医药》2013年第8期793-795,I0002,共4页Tianjin Medical Journal

基　　金：辽宁省自然科学基金资助项目(项目编号:2013022067);辽宁省教育厅科学技术研究项目(项目编号:L2012307)

摘　　要：目的观察金属硫蛋白-1(MT-1)在小鼠肾脏各部位的表达及细胞内定位。方法选取成年雄性CD-1小鼠,剖腹取出肾脏,应用Western blot法检测小鼠肾皮质和髓质中MT-1蛋白的表达;应用免疫组织化学和免疫荧光技术观察MT-1在小鼠肾小管和集合管的定位;应用免疫荧光双标共聚焦显微扫描技术观察MT-1在小鼠肾小管和集合管上皮细胞内的亚细胞定位。结果 MT-1在小鼠肾脏皮质和髓质均有表达。在皮质迷路,主要表达于近曲小管和远曲小管;在髓放线和髓质,主要表达于近直小管、远直小管和集合管。近端小管表达最强,远端小管次之,集合管最弱。在近端小管位于上皮细胞游离面刷状缘及胞浆内;在远端小管位于上皮细胞胞浆内;在集合管位于上皮细胞基底侧。亚细胞定位在溶酶体。结论 MT-1在小鼠肾脏近端小管、远端小管和集合管的细胞定位不同,可能决定了其在肾脏各结构中发挥的作用是不同的。Objective To investigate the expression and cellular localization of metallothionein- 1 （MT- 1） in mouse kidney. Methods The adult male CD- 1 mice were selected as experimental animals. The protein of MT- 1 in mouse renal cortex and medulla was detected by Western blot assay. Immunohistochemistry and immunofluorescence methods were ap- plied to observe the location of MT- 1 in renal tubules and collecting ducts of mouse kidney. Double-immunofluorescence and coufocal laser scanning microscopy were used to observe the subcellular localization of MT- 1 in the epithelial cells of re- nal tubules and collecting ducts of mouse kidney. Results The expression of MT- 1 was detected in cortex and medulla of mouse kidney. The expression of MT-lwas mainly found in proximal convoluted tubules and distal convoluted tubules of cor- tical labyrinth, and proximal straight tubules, distal straight tubules and collecting ducts of ray medullary and medulla of kid- ney. The strongest expression of MT- 1 was found in proximal tubules, secondary in distal tubules and last in collecting ducts. The intracellular location of MT- 1 was in brush border of proximal tubules and intracytoplasm, in the distal tubule epi- thelial cell cytoplasm and in the basolateral membranes of collecting duct epithelial cells. The subcellular localization was found in the lysosome. Conclusion The different locations of MT- 1 expressed in proximal tubules, distal tubules and col- lecting ducts may determine different roles of them in kidney.

关 键 词：金属硫蛋白 肾 免疫组织化学 小鼠 近交ICR 

分 类 号：R739.5[医药卫生—肿瘤]