氩氦冷冻治疗9L/Fischer344大鼠脑胶质瘤模型的病理学研究  

Pathology research of 9L gliomas in Fischer344 rats treated by argon-helium cryosurgery

作　　者：刘剑[1] 马国亮[2] 张世忠[3] 林健[1] 黄传平[1] 林武鹏[1] 

机构地区：[1]解放军第四二一医院神经外科,广州510318 [2]解放军第四二一医院医院办公室,广州510318 [3]南方医科大学珠江医院神经外科,广州510282

出　　处：《中华神经医学杂志》2013年第8期783-786,共4页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（81172416）

摘　　要：目的 探讨氩氦冷冻治疗9L/Fischer344大鼠脑胶质瘤模型的病理改变. 方法 种植9L胶质瘤细胞于30只Fischer344大鼠鼠背建立9L/Fischer344大鼠脑胶质瘤模型,并按随机数字表法分为空白对照组（自然生长,不进行任何处理）和氩氦冷冻组（给予氩氦冷冻治疗）.接种后30d取出肿瘤组织进行病理学观察,应用HE染色、电镜下观察冷冻消融的肿瘤组织形态,应用免疫组织化学染色检测胶质纤维酸性蛋白（GFAP）和S-100蛋白表达. 结果 （1）光镜下空白对照组肿瘤细胞巢状排列紧密,肿瘤无包膜,瘤周小血管扩张,可见肿瘤细胞沿小血管浸润生长；瘤内新生血管丰富,瘤内常见出血、凝固性坏死灶.高倍镜下肿瘤细胞形态多样,核大深染、多核,异型性高.肿瘤细胞GFAP、S-100染色阴性,肿瘤细胞间胶质网可见阳性染色.（2）氩氦冷冻组HE染色显示冷冻中央区呈均匀性凝固性坏死,边缘区的炎性反应带主要为一些细胞碎片及大量的红、白细胞浸润：微血管内也发现大量的红细胞、血小板集合和血栓形成,并可见灶性出血.电镜显示冷冻区中央、边缘区组织细胞全部坏死或凋亡. 结论 氩氦刀可能是治疗胶质瘤有效的方法.Objective To research the pathological features of 9L/Fischer344 of rat gliomas treated by argon helium cryotherapy.Methods The 9L gliomas were planted into 30 Fischer344 rats to establish the 9L/Fischer344 rat models; these rat models were randomly divided into blank control group （not given any treatment） and argon helium cryotherapy group.Thirty d after that,tumor histopathology was observed; HE staining and electron microscopy were employed to observe the morphology of the tumors; immunohistochemical staining was used to detect the GFAP and S-100 protein expressions.Results In the blank control group,light microscope showed gliomas closely nested arrangement without envelope,minute vessels expanding in the surrounding area of the gliomas,and gliomas growing following the minute vessels; abundant neovascularization,hemorrhage and coagulated necrosis in the gloms were noted; At high magnification,morphological diversity,low differentiated degree,big and excessive nucleus with deep staining were noted in the gliomas; GFAP and S-100 preotein was negatively expressed in the gliomas cell,but positive expression was noted in the glial network between gliomas.In the argon helium cryotherapy group,uniformed coagulation necrosis was noted in the central area,and cell debris and a lot of red and leukocyte infiltration were noted in the marginal zone; a large number of red blood cells,platelets and thrombosis,and visible focal hemorrhage were noted in the capillaries;tissue necrosis or cell apoptosis was noted in all central and marginal zones.Conclusion Argon helium cryotherapy might be an effective way for treating gliomas.

关 键 词：氩氦冷冻 9L胶质瘤细胞 Fischer344大鼠 病理学 

分 类 号：R739.41[医药卫生—肿瘤]