检测肺癌患者血清Cathepsin X及Cystatin C的临床意义  

Clinical Significance of Detection of Cathepsin X and Cystatin C in the Sera of Patients with Lung Cancer

作　　者：张学德[1] 侯彦丽[1] 牛泽群[1] 李维[1] 孟夏[1] 张娜[1] 杨拴盈[1] 

机构地区：[1]西安交通大学第二附属医院呼吸科,西安710004

出　　处：《中国肺癌杂志》2013年第8期411-416,共6页Chinese Journal of Lung Cancer

基　　金：国家自然科学基金资助项目(No.81172234)资助~~

摘　　要：背景与目的组织蛋白酶X(Cathepsin X,Cat X)是最近发现的一种组织蛋白酶(Cathepsins,Cats)家族成员。近年来研究表明CatX与多种恶性肿瘤发生、发展有关。本研究旨在探讨肺癌患者血清CatX及cystatinC的表达与临床特征及预后的关系。方法采用ELISA法定量检测84例肺癌患者及36例健康对照者血清CatX及cystatinC表达。结果肺癌患者血清Cat X和cystatin C水平明显高于健康人(P<0.01);CatX水平与肺癌病理类型之间有相关的趋势(P=0.076)。血清cystatin C水平与肺癌TNM分期正相关(P=0.01),cystatinC/CatX与淋巴结转移之间有相关趋势(P=0.058)。CatX表达水平与肺癌患者总生存期(overallsurvival,OS)相关,高水平CatX肺癌患者OS更短。Cox单因素回归示CatX高表达以及TNM分期是影响肺癌预后独立因素,Cox多因素回归显示,仅TNM分期是患者预后的独立危险因素。结论肺癌患者中血清CatX和cystatinC水平升高,检测肺癌患者Cat X和cystatin C血清水平对于指导临床肺癌诊断、评估预后有重要意义。Background and objective Cathepsin X （Cat X） has been identified as a member of cathepsin family. Studies have shown that Cat X is involved in tumorigenesis and tumor development of various cancers. ＂Ihe aim of this study is to investigate the relationship between the clinicopathological prognosis and the levels of Cat X and cystatin C in the serum of patients with lung cancer. Methods Blood samples were collected from 84 patients with lung cancer and 36 healthy control subjects. Cat X and cystatin C were determined by quantitative ELISA. Results Cat X and cystatin C levels were significantly higher in the patients with lung cancer than that in the healthy control subjects （P〈0.01）. Cat X level was correlated with the pathological types of lung cancer （P=0.076）. Cystatin C was positively correlated with TNM stage （P=0.01）. Furthermore, cystatin C/Cat X was correlated with lymph node metastasis （P=0.058）. The patients with high Cat X levels experienced sig- nificantly shorter overall survival rates compared with those with low Cat X. Univariate analysis indicated that Cat X and TNM stage were related to overall survival. Multivariate Cox analysis indicated that TNM stage may be used as an independent prog- nostic variable in patients with lung cancer. Conclusion Cat X and cystatin C levels were significantly higher in patients with lung cancer. Cat X and cystatin C detection in the sera may contribute to the diagnosis of lung cancer and may be used to evalu- ate the prognosis of patients with NSCLC.

关 键 词：CATHEPSIN X CYSTATIN C 肺肿瘤 血清 

分 类 号：R734.2[医药卫生—肿瘤]