MicroRNA-218在肝细胞癌中的表达及功能  

MicroRNA-218 expression and its role in hepatocellular carcinoma

作　　者：李超[1] 涂康生[1] 郑鑫[1] 张竞[1] 拓航[1] 高洁[1] 姚英民[1] 刘青光[1] 

机构地区：[1]西安交通大学医学院第一附属医院肝胆外科,陕西西安710061

出　　处：《南方医科大学学报》2013年第8期1127-1131,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81272645;81071897)~~

摘　　要：目的检测MicroRNA-218(miR-218)在肝细胞癌中的表达情况并研究其在肝癌中的功能。方法收集46例肝细胞癌及对应癌旁组织,运用qRT-PCR技术检测miR-218在肝癌及对应癌旁组织中的表达情况;应用miR-218 mimic转染人肝癌细胞系HepG2,MTT和流式细胞术检测转染后细胞活力和凋亡变化,qRT-PCR和Western blot检测miR-218潜在靶点的表达变化。结果 miR-218在肝癌组织中的表达显著低于对应的癌旁组织(P<0.05);miR-218低表达与肿瘤大小(>5 cm)和高TNM分期(Ⅲ+Ⅳ)具有显著相关性(P<0.05);在HepG2细胞中,miR-218抑制细胞增殖和诱导凋亡,并下调Bmi-1和CDK6 mRNA及蛋白表达(P<0.05)。结论 miR-218低表达与肝癌的恶性临床病理特征相关,miR-218可能通过下调Bmi-1和CDK6表达抑制肝癌细胞增殖和促进凋亡。Objective To investigate the expression of microRNA-218(miR-218) and its role in hepatocellular carcinoma(HCC).Methods Forty-six pairs of fresh surgical specimens of HCC and adjacent tissues were examined for miR-218 expression using qRT-PCR.A miR-218 mimic was transfected into HepG2 cells,and the cell viability and apoptosis were analyzed by MTT assay and flow cytometry,and the potential targets of miR-218 were detected by qRT-PCR and Western blotting.Results The expressions of miR-218 in HCC tissues were significantly down-regulated compared to those in the adjacent tissues(P<0.05).Down-regulation of miR-218 was found to correlate significantly with the tumor size(>5 cm) and an advanced TNM stage(III+ IV)(P<0.05).Ectopic expression of miR-218 in HepG2 cells resulted in suppressed cell proliferation and enhanced cell apoptosis as well as the down-regulation of Bmi-1 and CDK6 mRNA and protein expressions(P<0.05).Conclusion The lowexpression of miR-218 is correlated with malignant clinicopathological characteristics of HCC,and miR-218 may inhibit cell proliferation and promote cell apoptosis by down-regulating Bmi-1 and CDK6 in HCC.

关 键 词：MicroRNA-218 肝细胞癌 凋亡 增殖 BMI-1 CDK6 

分 类 号：R735.7[医药卫生—肿瘤]