食管鳞癌组织p16与Ki-67表达临床意义分析  

Clinical significance of expression of p16,Ki-67 in esophageal squamous cell carcinoma tissues

作　　者：孙爱民 李道堂[2] 穆殿斌[3] 孙诚 李波波[2] 

机构地区：[1]青岛市城阳人民医院肿瘤科,山东青岛266109 [2]山东省肿瘤医院胸外科,山东济南250117 [3]山东省肿瘤医院病理科,山东济南250117

出　　处：《中华肿瘤防治杂志》2013年第16期1236-1239,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:探讨食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)组织中p16与Ki-67表达,分析其与临床病理特征及预后相关性。方法:采用免疫组织化学SP法检测63例术后ESCC组织中p16与Ki-67表达,并分析与临床病理特征及预后的关系。表达程度与临床病理参数之间应用Spearman相关分析处理,应用Cox比例风险模型作预后因素分析。结果:ESCC组织中p16缺失率和Ki-67的阳性表达增高率分别为58.73%(37/63)和65.08%(41/63),p16缺失和Ki-67表达增高呈正相关,P=0.001。p16缺失及Ki-67阳性表达与临床分期(P值均<0.001)、淋巴转移(P值分别为0.010、0.008)呈正相关,而与肿瘤大小(P值分别为0.396和0.098)及组织学分级(P值分别为0.514和0.389)无关。Kap-lan-Meier生存分析结果显示,p16表达缺失率(P=0.004)及Ki-67的表达增高率(P=0.006)与生存时间缩短呈正相关。多因素Cox回归分析显示,p16(P=0.038)和Ki-67(P=0.044)表达、淋巴转移(P=0.021)、临床分期(P=0.031)和术后治疗(P=0.004)是影响患者预后的独立危险因素。结论:食管鳞状细胞癌组织中p16与Ki-67表达与临床分期、淋巴结转移和预后相关。p16与Ki-67检测有可能成为判断食管鳞状细胞癌分期和预后的重要分子标志,并成为重要的预后因素。OBJECTIVE：To elucidate the expression of p16, Ki 67 in Esophageal Squamous Cell Carcinoma（ESCC） and its relationship with clinicopathologic characteristics and prognosis of patients of ESCC. METHODS：Expressions of p16 and Ki 67 were determined by SP imunohistochemical technique in 63 consecutive patients with ESCC. The prognosis of ESCC was evaluated by Kaplan-Meier survival analysis, and the relationship between expression of p16 and Ki-67 and clinicopathological indices were analyzed by statistics. RESULTS：The loss rate of p16 and the positive rate of Ki-67 were 58. 730/00（37/63） ,65.08~/00（41/63） ,respectively. The loss expression of the p16 and the high expression of Ki-67 was pos- itive correlation（P~ 0. 001）, and it had a positive correlation with clinical stage（P~ 0. 001） and lymphnode metastasis （P were 0. 010 and 0. 008） too. It was no relation with tumor size （P were 0. 396 and 0. 098） and histological grading （P were 0. 514 and 0. 389）. Kaplan-Meier survival analysis curve displayed that the p16 expression rate and Ki-67 in- creasing rate and survival time were positively correlated （P^0.01）. Multivariate Cox regression analysis showed that p16 （P=0. 038） and Ki-67 （P=0. 044） expression,lymph node metastasis （P=0. 021）,clinical staging （P=0. 031）, postoperative treatment （P= 0. 004） were the independent risk factors of prognosis of patients. CONCLUSIONS：In esoph- ageal squamous cell carcinoma the loss of pl 6 expression and Ki-67 expression increase with the clinical staging, prognosis and lymph node metastasis of the disease, p16 and Ki-67 detection may be used in esophageal squamous cell carcinoma in staging and as important prognostic molecular markers.

关 键 词：食管肿瘤 癌 鳞状细胞 P16 KI-67 预后 

分 类 号：R735.1[医药卫生—肿瘤]