传染性法氏囊病病毒四川分离株VP2基因序列分析  

Sequence Analysis of VP2 Gene of Infectious Bursal Disease Virus Isolates from Sichuan Province

作　　者：严专强[1] 刘迪[1,2] 刘佳佳[1] 覃健萍[2] 鲁俊鹏[2] 谢青梅[1] 毕英佐[1] 陈峰[1] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642 [2]广东温氏食品集团股份有限公司,广东云浮527439

出　　处：《动物医学进展》2013年第8期18-23,共6页Progress In Veterinary Medicine

基　　金：广东省产学研资助项目(2010B090301019)

摘　　要：为了解四川地区传染性法氏囊病病毒(IBDV)的变异规律,本研究对2011年分离自四川的10个毒株进行了VP2基因高变区的克隆与测序。序列分析表明,10个分离株之间核苷酸序列同源性在95.3%～99.9%之间,其推导氨基酸序列同源性在99.2%～100%之间;所有分离株与国内外IBDV超强毒株(HK46、OKYM、UK661)的核苷酸同源性在94.4%～98.5%之间,其推导氨基酸序列同源性在98.6%～100%之间。遗传进化分析表明,分离株与超强毒株属同一分支,亲缘关系较近,并且与超强毒株VP2高变区内的特征性氨基酸相符合,据此推测四川省目前流行的毒株仍以超强毒株为主。本研究分离株与常用疫苗株的亲缘关系较远,一定程度上反映了当前四川省IBDV流行毒株与疫苗毒株之间的进化距离。To investigate the variation of infectious bursal disease viruses（IBDV）in Sichuan province, the VP2 hypervariable region of ten isolates in Sichuan in 2011 were cloned and sequenced. The results showed that,the VP2 genes of all isolates shared a 95.3% to 99.9% nucleotide homology, while shared a homolo- gy of 94.4% to 98.5% with the vvIBDV strains（HK46,OKYM,UK661）. These strains also shared an a- mino acid identity ranging from 99.2% to 100%. While compared with the vvIBDV strains,the range was 98.6 % to 100%. Phylogenetic analysis indicated that all the isolates were highly related with the vvIBDV strains, and the amino acid characteristic of VP2 hypervariable regions was the same as vvIBDV strains. This result showed that vvIBDV was still the epidemic type of virus in Sichuan province. The article dem- onstrated the isolates had less related with the vaccine strains, which revealed that the current epidemic strains had genetic distances with the vaccine strains.

关 键 词：传染性法氏囊病病毒 VP2基因高变区 克隆 序列分析 

分 类 号：S852.659.4[农业科学—基础兽医学] Q789[农业科学—兽医学]