硫化氢在肝硬化大鼠肝脏缺血再灌注损伤中的保护作用  

Protective Effect of Hydrogen Sulfide on Hepatic Ischemia-reperfusion Injury in Rats with Liver Cirrhosis

作　　者：牟燕[1] 魏来[1] 陈文雁[1] 王雯婷[1] 刘芝[1] 孔高茵[1] 

机构地区：[1]湖南省人民医院,湖南长沙410005

出　　处：《医学临床研究》2013年第7期1292-1293,1296,共3页Journal of Clinical Research

摘　　要：【目的】研究硫化氢（NaHS）预处理对肝硬化大鼠肝脏缺血再灌注后肝功能变化的影响，并探讨线粒体ATP敏感性钾通道（mitoKATP）在其中发挥的作用。【方法】32只肝硬化大鼠随机分为四组（n=8），包括假手术组（Sham组），肝脏缺血再灌注损伤组（HIRI组），NaHS预处理组（NaHS组）和NaHS＋mitoK—ATP通道阻断剂预处理组（5-HD组）。再灌注末取腔静脉血检测并比较肝功能指标：总胆红素（TBIL）、直接胆红素（DBIL）、谷丙转氨酶（ALT）和谷草转氨酶（AST）浓度。【结果】再灌注末，HIRI组、NaHS组和5一HD组TBIL、DBIL、ALT和AST浓度明显高于Sham组，HIRI组和5HD组无明显差异，NariS组低于HIRI组和5-HD组（P〈0．05）。【结论】NaHS预处理能提高肝硬化大鼠抵抗HIRI的能力，可能是通过开放线粒体ATP敏感性钾通道实现的。[Objective]To study the effect of sodium hydrosulfide（NaHS, a donor of hydrogen sulfide） precondi- tioning on liver function change after hepatic ischemia-reperfusion（IR） injury in rats with liver cirrhosis, and explore the role of mitochondrial ATP-sensitive postassium channel（mitoK-ATP ） in it. [Methods]Thirty two rats with liver cirrhosis were randomly divided into four groups（ n = 8） including sham operated group（sham group）, hepatic IR in- jury group（HIRI group）, NariS preconditioning group （NariS group） and NariS q- mitoK-ATP group （group 5- HD）. The inferior vena cava blood were collected at the end of reperfusion and liver function indexes such as total bil- irubin（TBIL）, direct bilirubin（DBIL）, alanine transaminase（ALT） and aspartate aminotransferase（AST） were com- pared. [Results]At the end of reperfusion, TBIL, DBIL,ALT and AST in group HIRI, group Naris and group 5- HD were obviously higher than those in sham group. There was no significant difference between group HIRI and group 5-HD. The indexes of group Naris were lower than those in group HIRI and group 5-HD（ P 〈0. 05）. [Conclusion]NariS preconditioning can improve the ability of liver cirrhosis rats against hepatic IR injury, which may be completed by opening mitoK-ATP .

关 键 词：肝硬化 再灌注损伤 大鼠 

分 类 号：G657.31[文化科学—教育学]