聚乙烯磷酸对牙本质基质金属蛋白酶活性的影响  

The influence of polyvinylphosphonic acid on matrix metalloproteinases activities in dentin

作　　者：何恩宝[1] 王彦[1] 梁澜晨[1] 王烟岚[1] 林雪峰[1] 

机构地区：[1]中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室,广州510055

出　　处：《中华口腔医学研究杂志（电子版）》2013年第4期21-24,共4页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：2012年广东省大学生创新训练项目(1055812391)

摘　　要：目的研究聚乙烯磷酸(PVPA)对牙本质中由酸蚀激活的基质金属蛋白酶(MMPs)的作用。方法收集50颗口腔颌面外科拔除的人正常第三磨牙,制备成牙本质粉并随机平均分成8组,对照组仅37%磷酸处理,实验组37%磷酸处理后分别用100、200、500、1000或3000μg/mlPVPA处理,阳性对照组为37%磷酸处理后用0.2%或2%氯己定处理。提取牙本质总蛋白,分别检测MMPs总活性、MMP-2、MMP-8和MMP-9活性。结果各实验浓度的PVPA均可有效抑制牙本质中被磷酸激活的MMPs总活性,除了100μg/mlPVPA处理组未出现明显的MMP-8活性抑制作用以外,其它各实验组PVPA均可有效抑制MMP-2、MMP-8和MMP-9的活性,且抑制作用随浓度升高而增强。结论 PVPA可有效抑制牙本质中由于酸蚀激活的MMP-2、MMP-8、MMP-9的活性,且抑制作用随浓度升高而增强。Objective The purpose of this study was to test the effect of polyvinylphosphonic acid （PVPA） which is used in biomimetic remineralization on dentin matrix metalloproteinases-2 （MMP- 2）, MMP-8, MMP-9 activated by etching treatment. Methods In this study, 50 extracted non-caries human third molars were used to be pulverized and then divided into 8 groups as follows： negative control group only treated with 37% H3PO4; experimental groups： incubated in 100,200, 500, 1000 or 3000μg/ml of PVPA after treated with 37% H3PO4, respectively; positive control groups： incubated in 0.2% or 2% chlorhexidine after treated with 37% H3PO4. The total protein was extracted, and the activities of MMPs, MMP-2, MMP-8 and MMP-9 were determined. Results All tested PVPA concentrations were highly effective in inhibiting MMPs. All tested PYPA concentrations show potent inhibitory effect on MMP-2, MMP-8 and MMP-9 activity except the 100 μg/ml PVPA treated group on MMP-8 activity. And a higher concentration of PVPA caused an increase in inhibitory effect. Conclusion PVPA is relatively effective in inhibiting MMPs and the inhibitory effect enhance with increasing PVPA concentration

关 键 词：聚乙烯磷酸 基质金属蛋白酶 牙本质 

分 类 号：R78[医药卫生—口腔医学]