四逆汤喷干粉中乌头生物碱大鼠体内吸收动力学研究  

Studies on absorption kinetics characteristics of diterpenoid alkaloids in rats after oral administration of Sini Tang powder

作　　者：吕长江[1] 王国伟[2] 戴东波[2] 李范珠[2] 陈静[2] 魏颖慧[2] 

机构地区：[1]杭州市红十字会医院制剂室,浙江杭州310003 [2]浙江中医药大学药学院,浙江杭州310053

出　　处：《中国中药杂志》2013年第14期2384-2388,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(90409012);浙江省自然科学基金项目(Y2091218);教育部博士基金项目(20103322120002);浙江省重点科技创新团队项目(2012R1004405)

摘　　要：研究四逆汤复方环境中乌头生物碱的体内吸收动力学特征。运用大鼠肝门静脉和颈静脉双位点插管技术取血,采用HPLC测定血浆中乌头碱、新乌头碱、次乌头碱,求算表观吸收系数,分析四逆汤喷干粉中3种乌头生物碱在大鼠体内的吸收动力学特征。结果显示,四逆汤喷干粉中新乌头碱、乌头碱、次乌头碱的表观吸收系数分别为0.336,0.090,0.176,新乌头碱的体内吸收程度优于乌头碱和次乌头碱。结果表明,肝门静脉和颈静脉双位点插管技术是一种研究药物肠道吸收的有效方法。大鼠灌胃四逆汤喷干粉后,3种乌头生物碱均可吸收入血,但吸收程度有明显差异。The purpose of this study was to investigate the absorption kinetics of aconitine,mesaconitine and hypaconitine in rats after oral administration of Sini Tang powder.With cannulate portal and jugular veins cannulated（double-cannulate）,conscious moving rats were orally administered Sini Tang.Then samples of portal and systemic blood were collected at the designated periods of time and analyzed for aconitine,mesaconitine and hypaconitine by HPLC.Apparent absorption coefficient of aconitine,mesaconitine and hypaconitine was caculated respectively.The results indicated that the apparent absorption coefficient of aconitine,mesaconitine and hypaconitine come from Sini Tang were 0.336,0.090,0.176,respectively,which had some differences among them.It was also suggested that double-cannulated rat was useful for estimating the absorption kinetics of aconitine,mesaconitine and hypaconitine after orally administered Sini Tang by determining the AUC values for drugs in portal and systemic blood samples.The three alkaloids could all be detected in blood,but the absorption differences were existed among the three alkaloids.

关 键 词：四逆汤 喷雾干燥 乌头生物碱 吸收动力学 双位点插管 大鼠 

分 类 号：R284[医药卫生—中药学]