碱提工艺对茶树菇多糖形貌特征和构象及其生物活性的影响  

Morphology and conformation of polysaccharide from Agrocybe aegerita and its antioxidant activity

作　　者：窦佩娟[1] 张静[2] 吕青青[3] 郭琦[1] 郭国赟[1] 孙润广[1] 

机构地区：[1]陕西师范大学物理学与信息技术学院生物物理与生物医学工程实验室,西安710062 [2]陕西师范大学食品工程与营养科学学院,西安710062 [3]武汉科技大学化学工程与技术学院,武汉430081

出　　处：《生物加工过程》2013年第4期22-30,共9页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(10874108);陕西省自然科学基础研究计划(SJ08A16)

摘　　要：利用传统水提及碱提的方法得到茶树菇粗多糖S-ACP和J-ACP,经CTAB法和Sephadex G-150凝胶层析法对其分离纯化,分别得到S-ACP2-1和S-ACP2-2以及J-ACP2-1和J-ACP2-2两组主要组分,用扫描电子显微镜(SEM)和原子力显微镜(AFM)对多糖的形貌进行表征并测定其体外抗氧化活性和抗肿瘤活性;对多糖S-ACP2-2、J-ACP2-2进行刚果红实验测定及圆二色谱仪(CD)分析。SEM观测结果:S-ACP2-1为较粗的表面光滑的丝状,J-ACP2-1呈较细的有少量碎屑的丝状;S-ACP2-2为较大的片状,J-ACP2-2在大的片状周围有很多细小的碎屑。AFM观测结果:碱液可以使多糖分子部分断裂成小片段。刚果红实验:S-ACP2-2、J-ACP2-2在水溶液中为自由卷曲构型。CD分析:S-ACP2-2的空间构型中有序结构较少,J-ACP2-2在水溶液中为无序构型。对比4种多糖的活性,碱液作用的多糖J-ACP2-2活性高于S-ACP2-2。A polysaccharide named S-ACP was extracted from Agrocybe aegerita by hot water extraction,after that, a polysaccharide named J-ACP was isolated from the dried leaf mustard with alkali. The crude polysaccharides were purified by CTAB and Sephadex G-150 in sequence, and the obtained ones were designated as S-ACP2-1, S-ACP2-2 ,J-ACP2-1 and J-ACP2-2 ,respectively. SEM and AFM were used in characterizing the morphology of the polysaccharide, and AFM was used to observe in vitro antioxidant and anti-tumor activities. Polysaccharide S-ACP2-2 and J-ACP2-2 were used for Congo red test and CD analysis. SEM showed that S-ACP2-1 and J-ACP2-1 existed in filamentous form, S-ACP2-2 and J-ACP2-2 existed in sheet form, S-ACP2-1 had stronger rigidity than J-ACP2-1, and the sheet of S-ACP2-2 was bigger than that of J-ACP2-2. AFM images showed that： the alkali could make a portion of the polysaccharide molecule break into small pieces. The conformations of S-ACP2-2 and J-ACP2-2 were studied by complex-formation with Congo red, result showed that the conformations were mainly in disorder coli in water solution. The CD spectrum showed that only a little of ACP2-2 was in ordered structure in the spatial configuration and J-ACP2-2 was in disordered conformation in aqueous solution. Comparison of activities of four kinds of polysaccharides, J-ACP2-2 treated with alkaline had stronger activity.

关 键 词：茶树菇 圆二色谱 显微镜 多糖 

分 类 号：S646.19[农业科学—蔬菜学] Q539[农业科学—园艺学]