PilC蛋白对人尿道上皮细胞STAT3激活及TNF-α、IL-8分泌的影响  

The impact of PilC protein on the activation of THUECs cell line STAT3 and the secretion of TNF-α and IL-8

作　　者：连昕[1] 周宇[1] 涂亚庭[1] 林能兴[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤科,武汉430022

出　　处：《中国麻风皮肤病杂志》2013年第8期493-495,共3页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金(编号:81171726)

摘　　要：目的:评价PilC蛋白对人尿道上皮细胞(THUECs细胞)STAT3基因及其下游细胞因子TNF-α和IL-8表达的影响。方法:采用细胞内染色法检测PilC蛋白刺激后THUECs细胞系-磷酸化STAT3(p-STAT3)表达;ELISA法检测细胞培养上清中TNF-α和IL-8水平。结果:PilC蛋白刺激后,细胞内p-STAT3表达升高,且与PilC蛋白浓度成正比,并能增强TNF-α和IL-8的分泌。结论:PiIC蛋白能增强THUECs细胞系内p-STAT3表达和明显增加TNF-α和IL-8水平。Objective：To evaluate the impact of PilC protein on STAT3 gene, and TNF-α and IL-8 ex- pression in urethral epithelial cells （THUECs）. Methods： After the THUECs cells were stimulated by PilC protein, the expression of THUECs cells phosphory]ation STAT3 （ P - STAT3 ） was detected by intracellular staining method; TNF-α, IL-8 levels in the supernatant were detected by ELISA.Results： After the cells were stimulated by PilC protein, the intracellular p-STAT3 expression increased, which was positively corre- lated to the concentration of PilC protein. Apart from this, the levels of TNF-α and IL-8 in the supernatant were also elevated. Conclusion： PilC protein can enhance the expression of THUECs cell p-STAT3, and can increase the secretion of TNF-α and IL-8 in cultural supernatant.

关 键 词：PilC蛋白 THUECs细胞系 信号传导与转录激活因子3 肿瘤坏死因子-Α 白介素-8 

分 类 号：R363[医药卫生—病理学]