抗hAR-N段McAb的制备及其在免疫组化检测中的应用  

PREPARATION AND APPLICATION OF MONOCLONAL ANTIBODIES AGAINST hAR-N IN IMMUNOHISTOCHEMISTRY

作　　者：沈敬华[1] 唐振华[2] 赵利珍[3] 贾柄权[1] 李维才[4] 

机构地区：[1]内蒙古医学院微生物教研室 [2]内蒙古医学院教务处 [3]内蒙古医学院病理室 [4]内蒙古医学院医疗系

出　　处：《内蒙古医学院学报》2000年第3期150-152,共3页Acta Academiae Medicinae Neimongol

摘　　要：目的 :自行研制抗人雄激素受体 (androgenreceptor,AR)单克隆抗体 (McAb) ,以期达到可替代国外AR多克隆抗体。方法 :采用人雄激素受体合成肽 (hAR -N)交联钥孔戚血蓝素 (KLH)为抗原 ,免疫BALB/C小鼠 ,经用Kohler和Milstein杂交瘤技术成功地获得 4株能稳定分泌抗hAR单克隆抗体 (McAb)杂交瘤细胞株 5 5 ,141,2 0 7,2 5 7。结果 :对 45例前列腺癌组织石蜡切片进行了免疫组化 (IHC)检测 ,同时对自制抗hAR -NMcAb与进口抗AR多抗进行分析比较 ,阴阳总符合率为 91%。结论 :自制抗ARMcAb特异性强 ,灵敏度高 ,结果与Zymed公司产品相同 ,操作简便 ,适于临床应用。Objective:To study and prepare anti human androgen receptor(AR) monoclonal antibodies (McAb).Methods:Four cell lines of hybridoma secreting specific McAb against AR were first established by fusion SP2/0 cells with spleen cells from BALB/C mice immunized with the coupling complex of hAR-KLH.Results:Paraffin-embedded sections of 45 prostate cancers were detected.There was an overall concordance of 91% using IHC between AR ployclonal antibody from Zymed and hAR-N McAb in our work.Conclusion:The results show that the McAb obtained in this study would be a useful tool to detect the AR status in prostate cancer.

关 键 词：前列腺肿瘤 单克降抗体 免疫组织化学 

分 类 号：R737.250.3[医药卫生—肿瘤]